Human 11-Beta-Hydroxysteroid Dehydrogenase Type 1 (HSD11b1) ELISA Kit
The Human 11-beta-Hydroxysteroid Dehydrogenase Type 1 (HSD11B1) ELISA Kit is specially designed for the accurate quantification of HSD11B1 levels in human serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring precise and consistent results that are suitable for a broad range of research applications.HSD11B1 is a key enzyme involved in the interconversion of active and inactive forms of glucocorticoids, playing a crucial role in modulating cellular responses to stress and inflammation. Dysregulation of HSD11B1 has been implicated in various diseases, including metabolic disorders, obesity, and cardiovascular conditions, highlighting its significance as a biomarker for understanding these pathologies and exploring potential therapeutic interventions.
With its advanced technology and superior performance, the Human HSD11B1 ELISA Kit provides researchers with a valuable tool for studying the role of HSD11B1 in health and disease, ultimately contributing to the advancement of biomedical science and the development of novel treatment strategies.
Product Name:
Human 11-Beta-Hydroxysteroid Dehydrogenase Type 1 (HSD11b1) ELISA Kit
Product Code:
HUDL01370
Size:
96 Assays
Target:
HSD11b1
Synonyms:
11DH, 11-beta-HSD1, HDL, HSD11, HSD11B, HSD11L, SDR26C1, Corticosteroid 11-beta-dehydrogenase isozyme 1, Short Chain Dehydrogenase/Reductase Family 26C,Member 1
Detection Method:
Sandwich
Range:
0.156-10ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
83-99
91
EDTA plasma (n=5)
80-100
90
Heparin plasma (n=5)
82-99
90
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
