Human ACP1 Recombinant Protein (RPPB1336)
- SKU:
- RPPB1336
- Product Type:
- Recombinant Protein
- Species:
- Human
- Uniprot:
- P24666
- Research Area:
- Enzymes
Description
| Product Name: | Human ACP1 Recombinant Protein |
| Product Code: | RPPB1336 |
| Size: | 20µg |
| Species: | Human |
| Target: | ACP1 |
| Synonyms: | HAAP, MGC3499, MGC111030, ACP1, Low molecular weight phosphotyrosine protein phosphatase, LMW-PTPase, LMW-PTP, Low molecular weight cytosolic acid phosphatase, Red cell acid phosphatase 1, Adipocyte acid phosphatase. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile filtered colorless solution. |
| Formulation: | The ACP1 protein solution contains 20mM MES, pH-6, 0.1mM PMSF, 2mM EDTA and 10% Glycerol. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Purity: | Greater than 95.0% as determined by SDS-PAGE. |
| Amino Acid Sequence: | MGSSHHHHHH SSGLVPRGSH MAEQATKSVL FVCLGNICRS PIAEAVFRKL VTDQNISENW VIDSGAVSDW NVGRSPDPRA VSCLRNHGIHTAHKARQITK EDFATFDYIL CMDESNLRDL NRKSNQVKTC KAKIELLGSY DPQKQLIIED PYYGNDSDFE TVYQQCVRCC RAFLEKAH |
ACP1 is part of the phosphotyrosine protein. ACP1 functions as an acid phosphatase and a protein tyrosine phosphatase (PTPase) existing in all human tissues, including adipocytes. ACP1 enzyme hydrolyzes protein tyrosine phosphate to protein tyrosine and orthophosphate, and also orthophosphoric monoesters to alcohol and orthophosphate. ACP1 is present in adipocytes, thus playing a specific role in the regulation of adipose tissue. High levels of the ACP1 negatively regulate cell proliferation and growth of leiomyomas during dephosphorylation of the PDGF receptor. High significant differences in birth weight-placental weight relationships were observed among acid phosphatase locus 1 phenotypes.
Recombinant Human ACP1 produced in E.Coli is a single, non-glycosylated polypeptide chain containing 178 amino acids (1-158 a.a.) and having a molecular mass of 20.1 kDa. ACP1 is fused to a 20 amino acid His-Tag at N-Terminus and purified by conventional chromatography techniques.
| UniProt Protein Function: | ACP1: a phosphotyrosine protein phosphatase. Functions as an acid phosphatase and a protein tyrosine phosphatase by hydrolyzing protein tyrosine phosphate to protein tyrosine and orthophosphate. Hydrolyzes orthophosphoric monoesters to alcohol and orthophosphate. Genetically polymorphic with three common alleles segregating at the corresponding locus give rise to six phenotypes. Each allele appears to encode at least two electrophoretically different isozymes, Bf and Bs, which are produced in allele-specific ratios. Three transcript variants encoding distinct isoforms have been identified for this gene. |
| UniProt Protein Details: | Protein type:Cofactor and Vitamin Metabolism - riboflavin; EC 3.1.3.2; Protein phosphatase, tyrosine (non-receptor); Motility/polarity/chemotaxis; EC 3.1.3.48; Phosphatase (non-protein) Chromosomal Location of Human Ortholog: 2p25 Cellular Component: internal side of plasma membrane; cytoplasm Molecular Function:protein binding; acid phosphatase activity; non-membrane spanning protein tyrosine phosphatase activity |
| NCBI Summary: | The product of this gene belongs to the phosphotyrosine protein phosphatase family of proteins. It functions as an acid phosphatase and a protein tyrosine phosphatase by hydrolyzing protein tyrosine phosphate to protein tyrosine and orthophosphate. This enzyme also hydrolyzes orthophosphoric monoesters to alcohol and orthophosphate. This gene is genetically polymorphic, and three common alleles segregating at the corresponding locus give rise to six phenotypes. Each allele appears to encode at least two electrophoretically different isozymes, Bf and Bs, which are produced in allele-specific ratios. Multiple alternatively spliced transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq, Aug 2008] |
| UniProt Code: | P24666 |
| NCBI GenInfo Identifier: | 1709543 |
| NCBI Gene ID: | 52 |
| NCBI Accession: | P24666.3 |
| UniProt Related Accession: | P24666 |
| Molecular Weight: | Molecular Mass: 19.04kDaActual Protein�Molecular Mass: 18kDa |
| NCBI Full Name: | Low molecular weight phosphotyrosine protein phosphatase |
| NCBI Synonym Full Names: | acid phosphatase 1 |
| NCBI Official Symbol: | ACP1�� |
| NCBI Official Synonym Symbols: | HAAP; LMWPTP; LMW-PTP�� |
| NCBI Protein Information: | low molecular weight phosphotyrosine protein phosphatase |
| UniProt Protein Name: | Low molecular weight phosphotyrosine protein phosphatase |
| UniProt Synonym Protein Names: | Adipocyte acid phosphatase; Low molecular weight cytosolic acid phosphatase (EC:3.1.3.2); Red cell acid phosphatase 1 |
| Protein Family: | Low molecular weight phosphotyrosine protein phosphatase |
| UniProt Gene Name: | ACP1�� |
| UniProt Entry Name: | PPAC_HUMAN |
