The Human Apoptosis-Inducing Factor (AIF) ELISA Kit is a cutting-edge tool designed for the precise quantification of AIF levels in human samples, including serum, plasma, and cell culture supernatants. This advanced kit boasts exceptional sensitivity and accuracy, guaranteeing robust and consistent results for a diverse array of research applications.AIF is a key protein involved in apoptosis, or programmed cell death, playing a critical role in regulating cell survival and death pathways. Dysregulation of AIF has been linked to various diseases, including cancer, neurodegenerative disorders, and autoimmune conditions, highlighting its importance as a potential biomarker for disease diagnosis and therapeutic development.
With its user-friendly design and exceptional performance, the Human AIF ELISA Kit is a valuable asset for researchers studying apoptosis, cell death pathways, and the underlying mechanisms of various diseases. Trust in this reliable and innovative kit to accelerate your research and advance scientific discovery in the field of apoptosis biology.
Product Name:
Human Apoptosis Inducing Factor (AIF) ELISA Kit
Product Code:
HUDL00121
Size:
96 Assays
Target:
AIF
Synonyms:
PDCD8, Programmed Cell Death protein 8, Apoptosis-inducing factor 1, mitochondrial
Detection Method:
Sandwich
Range:
0.156-10ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-99
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
