The Human ASPN (Asporin) ELISA Kit is specifically developed for the precise measurement of asporin levels in human samples such as serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring accurate and consistent results for a variety of research purposes.Asporin is a key protein involved in regulating collagen deposition and bone formation, playing a crucial role in conditions like osteoarthritis and skeletal disorders.
Monitoring asporin levels can provide valuable insights into disease progression and therapeutic interventions, making this ELISA kit essential for researchers studying musculoskeletal conditions and bone health.
Product Name:
Human ASPN (Asporin) ELISA Kit
SKU:
HUES01712
Target:
Human ASPN (Asporin)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.19 ng/mL
Detection range:
0.31-20 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human ASPN. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human ASPN and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human ASPN, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human ASPN. You can calculate the concentration of Human ASPN in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
93-109
93-106
94-106
Average (%)
99
100
99
1:4
Range (%)
94-104
79-93
85-99
Average (%)
99
86
91
1:8
Range (%)
90-106
80-92
83-94
Average (%)
97
86
87
1:16
Range (%)
88-101
86-100
89-99
Average (%)
94
93
94
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
93-107
99
EDTA plasma (n=5)
91-106
98
Cell culture media (n=5)
95-111
101
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
1.05
2.51
9.49
1.12
2.65
8.77
Standard deviation
0.07
0.1
0.49
0.08
0.11
0.4
C V (%)
6.67
3.98
5.16
7.14
4.15
4.56
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human ASPN concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human ASPN in samples. No significant cross-reactivity or interference between Human ASPN and analogues was observed.
