Human ATPIF1 Recombinant Protein (RPPB5846)
- SKU:
- RPPB5846
- Product Type:
- Recombinant Protein
- Species:
- Human
- Uniprot:
- Q9UII2
Description
| Product Name: | Human ATPIF1 Recombinant Protein |
| Product Code: | RPPB5846 |
| Size: | 20µg |
| Species: | Human |
| Target: | ATPIF1 |
| Synonyms: | ATPase inhibitor mitochondrial, Inhibitor of F(1)F(o)-ATPase, IF(1), IF1, ATPIF1, ATPI, IP, ATPIP. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile Filtered colorless solution. |
| Formulation: | ATPIF1 protein solution (1mg/ml) containing 20mM Tris-HCl buffer (pH 8.0), 0.2M NaCl, 50% glycerol and 2mM DTT. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Purity: | Greater than 95.0% as determined by SDS-PAGE. |
| Amino Acid Sequence: | MGSSHHHHHH SSGLVPRGSH MGSHMGSDQS ENVDRGAGSI REAGGAFGKR EQAEEERYFR AQSREQLAAL KKHHEEEIVH HKKEIERLQK EIERHKQKIK MLKHDD |
ATPase inhibitory factor 1 (ATPIF1) attaches to the C-terminal region of a beta subunit of the F1-ATPase at low pH values and, through interference of the beta and gamma subunit interaction, ATPIF1 controls the activity of the F1 (the hydrophilic catalytic core), F0 (the membrane embedded protein channel) ATPase. ATPIF1 overexpression in a number of human carcinomas additionally reinforces its participation in oncogenesis and offers insight into the transformed metabolism of cancer cells, including the reprogramming of energy metabolism in relation to glycolysis. Endogenous F1F(o)-ATPase inhibitor curbing ATP depletion when the mitochondrial membrane potential drops below a threshold and the F1F(o)-ATP synthase begins hydrolyzing ATP to pump protons out of the mitochondrial matrix.
ATPIF1 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 106 amino acids (26-106 a.a) and having a molecular mass of 12.2kDa.ATPIF1 is fused to a 25 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
| UniProt Protein Function: | ATPIF1: Endogenous F(1)F(o)-ATPase inhibitor limiting ATP depletion when the mitochondrial membrane potential falls below a threshold and the F(1)F(o)-ATP synthase starts hydrolyzing ATP to pump protons out of the mitochondrial matrix. Homodimer at a pH value below 6.5, inactive homotetramer above. Belongs to the ATPase inhibitor family. 3 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Mitochondrial; Inhibitor Chromosomal Location of Human Ortholog: 1p35.3 Cellular Component: cell surface; mitochondrial proton-transporting ATP synthase complex; mitochondrion Molecular Function:5-formyltetrahydrofolate cyclo-ligase activity; angiostatin binding; ATPase binding; ATPase inhibitor activity; calmodulin binding; enzyme inhibitor activity; protein homodimerization activity Biological Process: angiogenesis; erythrocyte differentiation; folic acid and derivative biosynthetic process; generation of precursor metabolites and energy; heme biosynthetic process; mitochondrial depolarization; negative regulation of ATPase activity; negative regulation of endothelial cell proliferation; negative regulation of hydrolase activity; protein homooligomerization; protein homotetramerization |
| NCBI Summary: | This gene encodes a mitochondrial ATPase inhibitor. Alternative splicing occurs at this locus and three transcript variants encoding distinct isoforms have been identified. [provided by RefSeq, Jul 2008] |
| UniProt Code: | Q9UII2 |
| NCBI GenInfo Identifier: | 12585262 |
| NCBI Gene ID: | 93974 |
| NCBI Accession: | Q9UII2.1 |
| UniProt Secondary Accession: | Q9UII2,Q5JXL8, Q6IAQ7, Q9BSL9, |
| UniProt Related Accession: | Q9UII2 |
| Molecular Weight: | |
| NCBI Full Name: | ATPase inhibitor, mitochondrial |
| NCBI Synonym Full Names: | ATPase inhibitory factor 1 |
| NCBI Official Symbol: | ATPIF1�� |
| NCBI Official Synonym Symbols: | IP; ATPI; ATPIP�� |
| NCBI Protein Information: | ATPase inhibitor, mitochondrial |
| UniProt Protein Name: | ATPase inhibitor, mitochondrial |
| UniProt Synonym Protein Names: | Inhibitor of F(1)F(o)-ATPase; IF(1); IF1 |
| Protein Family: | ATPase inhibitor |
| UniProt Gene Name: | ATPIF1�� |
| UniProt Entry Name: | ATIF1_HUMAN |
