The Human AXIN2 (Axis Inhibition Protein 2) CLIA Kit is a cutting-edge tool for measuring levels of AXIN2 in human samples such as serum, plasma, and cell culture supernatants. This highly sensitive and specific kit delivers accurate and reproducible results, making it an invaluable asset for a variety of research applications.AXIN2 is a key player in the Wnt signaling pathway, regulating cell growth and development.
Dysregulation of AXIN2 has been linked to various diseases including cancer, osteoporosis, and developmental disorders, highlighting its importance as a potential biomarker for understanding disease mechanisms and exploring therapeutic interventions.Equip your research with the latest technology and insights by utilizing the Human AXIN2 CLIA Kit, advancing your understanding of AXIN2's role in health and disease.
Product Name:
Human AXIN2 (Axis Inhibition Protein 2) CLIA Kit
SKU:
HUES00375
Target:
Human AXIN2 (Axis Inhibition Protein 2)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
37.50 pg/mL
Detection range:
62.50-4000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human AXIN2. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human AXIN2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human AXIN2, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human AXIN2. You can calculate the concentration of Human AXIN2 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
84-99
87-102
93-106
Average (%)
90
93
100
1:4
Range (%)
86-102
88-102
94-111
Average (%)
93
94
102
1:8
Range (%)
94-108
99-111
96-109
Average (%)
99
105
102
1:16
Range (%)
92-103
96-110
89-102
Average (%)
97
104
95
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
89-103
95
EDTA plasma (n=5)
84-96
90
Cell culture media (n=5)
93-111
101
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
205.45
505.01
1362.3
217.66
501.87
1251.42
Standard deviation
23.57
51.66
84.19
17.67
52.6
77.46
C V (%)
11.47
10.23
6.18
8.12
10.48
6.19
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human AXIN2 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human AXIN2 in samples. No significant cross-reactivity or interference between Human AXIN2 and analogues was observed.
