Human BAFFR (B Cell Activating Factor Receptor) CLIA Kit
The Human BAFFR (B-Cell Activating Factor Receptor) CLIA Kit is a highly sensitive and specific assay designed for the accurate detection of BAFFR levels in human serum, plasma, and cell culture supernatants. This kit provides reliable and reproducible results, making it ideal for a wide range of research applications.BAFFR is a receptor protein that plays a crucial role in B-Cell activation and proliferation, making it a key target for studying immune responses and developing therapeutic interventions for autoimmune diseases, B-Cell malignancies, and other immune-related disorders.
This kit is essential for researchers looking to investigate the role of BAFFR in various disease conditions and potential therapeutic strategies.
Product Name:
Human BAFFR (B Cell Activating Factor Receptor) CLIA Kit
SKU:
HUES00377
Target:
Human BAFFR (B Cell Activating Factor Receptor)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human BAFFR. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human BAFFR and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human BAFFR, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human BAFFR. You can calculate the concentration of Human BAFFR in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
94-111
92-106
86-98
Average (%)
101
97
91
1:4
Range (%)
87-101
90-105
100-117
Average (%)
94
96
109
1:8
Range (%)
97-113
91-105
91-105
Average (%)
103
97
96
1:16
Range (%)
91-106
85-97
100-115
Average (%)
98
92
108
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
99-113
105
EDTA plasma (n=5)
88-103
94
Cell culture media (n=5)
89-104
95
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
97.02
308.33
944.42
105.7
315.38
886.97
Standard deviation
7.96
32.37
73.48
12.28
32.39
79.47
C V (%)
8.2
10.5
7.78
11.62
10.27
8.96
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human BAFFR concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human BAFFR in samples. No significant cross-reactivity or interference between Human BAFFR and analogues was observed.
