Human BANP (BTG3 Associated Nuclear Protein) CLIA Kit
The Human BANP (BTG3 Associated Nuclear Protein) CLIA Kit is a reliable and accurate tool for detecting BANP levels in human samples such as serum, plasma, and cell culture supernatants. With high sensitivity and specificity, this kit provides researchers with dependable and reproducible results for a variety of research applications.BANP is a key protein that plays a role in regulating gene expression and cell growth through its association with BTG3.
Dysregulation of BANP has been linked to various diseases, including cancer, making it a valuable biomarker for studying disease mechanisms and potential therapeutic targets.Overall, the Human BANP CLIA Kit offers a precise and efficient method for analyzing BANP levels in human samples, providing researchers with valuable insights into its role in disease progression and potential treatment strategies.
Product Name:
Human BANP (BTG3 Associated Nuclear Protein) CLIA Kit
SKU:
HUES01269
Target:
Human BANP (BTG3 Associated Nuclear Protein)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human BANP. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human BANP and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human BANP, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human BANP. You can calculate the concentration of Human BANP in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
90-104
90-104
94-106
Average (%)
97
97
100
1:4
Range (%)
95-107
96-113
101-120
Average (%)
100
103
109
1:8
Range (%)
84-100
100-116
100-115
Average (%)
91
106
105
1:16
Range (%)
88-100
94-107
97-115
Average (%)
94
99
105
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
84-99
91
EDTA plasma (n=5)
99-114
107
Cell culture media (n=5)
95-109
101
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
98.2
194.12
696.97
105.5
200.85
691.98
Standard deviation
8.02
20.52
77.22
9.22
17.88
79.23
C V (%)
8.17
10.57
11.08
8.74
8.9
11.45
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human BANP concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human BANP in samples. No significant cross-reactivity or interference between Human BANP and analogues was observed.
