Human BD 4 Recombinant Protein (RPPB0076)
- SKU:
- RPPB0076
- Product Type:
- Recombinant Protein
- Species:
- Human
- Uniprot:
- Q8WTQ1
- Research Area:
- Cytokines
Description
| Product Name: | Human BD 4 Recombinant Protein |
| Product Code: | RPPB0076 |
| Size: | 20µg |
| Species: | Human |
| Target: | BD 4 |
| Synonyms: | HBD-4, DEFB-4, HBD4, DEFB104B, Beta-defensin 4, BD-4. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile Filtered White lyophilized (freeze-dried) powder. |
| Formulation: | The DEFB4 (1mg/ml) was lyophilized with 20mM sodium Phosphate buffer pH-7.4 and 130mM NaCl. |
| Solubility: | It is recommended to reconstitute the lyophilized Beta Defensin-4 in sterile 18M?-cm H2O not less than 100�g/ml, which can then be further diluted to other aqueous solutions. |
| Stability: | Lyophilized Beta Defensin-4 Recombinant although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution BD-3 should be stored at 4°C between 2-7 days and for future use below -18°C.Please prevent freeze-thaw cycles. |
| Purity: | Greater than 98.0% as determined by:(a) Analysis by RP-HPLC.(b) Analysis by SDS-PAGE. |
| Amino Acid Sequence: | EFELDRICGY GTARCRKKCR SQEYRIGRCP NTYACCLRKW DESLLNRTKP |
| Biological Activity: | Determined by its ability to chemoattract human monocytes using a concentration range of 0.1-50 ng/ml, corresponding to a specific activity of 20,000-10,000,000 units/mg. |
Defensins are cationic peptides with a large spectrum of antimicrobial activity that comprise an important arm of the innate immune system. The Alpha defensins are differentiated from the Beta-defensins by the pairing of their 3 disulfide bonds. 4 human Beta-defensins have been identified to date; BD-1, BD-2, BD-3 and BD-4. Beta-defensins are expressed on some leukocytes and at epithelial surfaces. In addition to their direct antimicrobial activities, they are chemoattractant towards immature dendritic cells and memory T cells. The beta-defensin proteins are expressed as the C-terminal portion of precursors and are released by proteolytic cleavage of a signal sequence and, in the case of BD-1 (36 a.a.), a propeptide region. Beta-defensins contain a six-cysteine motif that forms three intra-molecular disulfide bonds. Beta-Defensins are 3-5 kDa peptides ranging in size from 33-47 amino acid residues.
Beta Defensin-4 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 50 amino acids and having a molecular mass of 6 kDa. The BD-4 is purified by proprietary chromatographic techniques.
| UniProt Protein Function: | defensin, beta 4: Has antimicrobial activity. Synergistic effects with lysozyme and DEFB103. Belongs to the beta-defensin family.Protein type: Secreted, signal peptide; SecretedChromosomal Location of Human Ortholog: 8p23.1Cellular Component: cell surface; extracellular regionBiological Process: defense response to bacterium; innate immune response |
| UniProt Protein Details: | |
| NCBI Summary: | Defensins form a family of antimicrobial and cytotoxic peptides made by neutrophils. Defensins are short, processed peptide molecules that are classified by structure into three groups: alpha-defensins, beta-defensins and theta-defensins. All beta-defensin genes are densely clustered in four to five syntenic chromosomal regions. Chromosome 8p23 contains at least two copies of the duplicated beta-defensin cluster. This duplication results in two identical copies of defensin, beta 104, DEFB104A and DEFB104B, in head-to-head orientation. This gene, DEFB104A, represents the more centromeric copy. [provided by RefSeq, Oct 2014] |
| UniProt Code: | Q8WTQ1 |
| NCBI GenInfo Identifier: | 118572637 |
| NCBI Gene ID: | 140596 |
| NCBI Accession: | Q8WTQ1.2 |
| UniProt Secondary Accession: | Q8WTQ1,Q496I2, Q496I3, Q496I4 |
| UniProt Related Accession: | Q8WTQ1 |
| Molecular Weight: | Approximately 150 kDa |
| NCBI Full Name: | Beta-defensin 104 |
| NCBI Synonym Full Names: | defensin, beta 104A |
| NCBI Official Symbol: | DEFB104A�� |
| NCBI Official Synonym Symbols: | BD-4; DEFB4; hBD-4; DEFB-4; DEFB104�� |
| NCBI Protein Information: | beta-defensin 104; defensin, beta 4 |
| UniProt Protein Name: | Beta-defensin 104 |
| UniProt Synonym Protein Names: | Beta-defensin 4; BD-4; DEFB-4; hBD-4; Defensin, beta 104 |
| Protein Family: | |
| UniProt Gene Name: | DEFB104A�� |
| UniProt Entry Name: | D104A_HUMAN |
