Human BMP Binding Endothelial Regulator (BMPER) ELISA Kit
The Human BMP Binding Endothelial Regulator (BMPER) ELISA Kit is a reliable tool for the accurate measurement of BMPER levels in human serum, plasma, and cell culture supernatants. This kit is specifically designed with high sensitivity and specificity to ensure precise and consistent results, making it an invaluable resource for a variety of research applications.BMPER is a key regulator of vascular development and plays a critical role in modulating the activity of bone morphogenetic proteins (BMPs) in the endothelial cells.
Dysregulation of BMPER expression has been linked to a range of diseases, including cardiovascular disorders and cancer, making it a valuable biomarker for studying these conditions and exploring potential therapeutic interventions.With the Human BMPER ELISA Kit, researchers can confidently investigate the role of BMPER in angiogenesis and vascular homeostasis, gaining valuable insights into the mechanisms underlying various diseases and paving the way for innovative treatment strategies.
Product Name:
Human BMP Binding Endothelial Regulator (BMPER) ELISA Kit
Product Code:
HUDL00346
Size:
96 Assays
Target:
BMPER
Synonyms:
Cv2, CRIM3, Crossveinless-2, Bone morphogenetic protein-binding endothelial cell precursor-derived regulator
Detection Method:
Sandwich
Range:
0.156-10ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
