The Human C3 (Complement Component 3) ELISA Kit is a cutting-edge assay meticulously tailored for the precise quantitative measurement of Complement Component 3 levels in diverse biological samples. C3 is a pivotal component of the complement system, essential for immune response modulation, inflammation regulation, and pathogen defense. As a key player in opsonization, chemotaxis, and the formation of the membrane-attack complex, C3 is integral to both innate and adaptive immune processes. This ELISA kit empowers researchers to elucidate the role of C3 in immune surveillance, inflammatory pathways, and various diseases. Accurate quantification of C3 is vital for unraveling its functions in immunity, autoimmune disorders, and infectious conditions. The C3 ELISA Kit provided by Assay Genie ensures exceptional sensitivity and specificity, guaranteeing precise and reproducible results. Crafted with stringent quality control measures, this kit delivers robust and reliable performance while remaining user-friendly. With Assay Genie's C3 ELISA Kit, researchers can confidently explore the intricacies of C3 involvement in immune responses and disease pathogenesis.
Product Name:
Human C3 (Complement Component 3) ELISA Kit
SKU:
AEES00090
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
0.94 ng/mL
Detection range:
1.56-100 ng/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
