Human Tumor Necrosis Factor Receptor Superfamily, Member 8 (TNFRSF8) ELISA Kit
The Human Tumor Necrosis Factor Receptor Superfamily Member 8 (TNFRSF8) ELISA Kit is a powerful tool for the accurate and reliable detection of TNFRSF8 levels in human samples such as serum, plasma, and cell culture supernatants. This kit offers superior sensitivity and specificity, ensuring precise and reproducible results that are essential for various research applications.TNFRSF8, also known as CD30, is a cell surface receptor belonging to the tumor necrosis factor receptor superfamily. It plays a critical role in regulating immune responses and cell survival.
Dysregulation of TNFRSF8 has been implicated in various diseases, including cancer, autoimmune disorders, and inflammatory conditions. Therefore, measuring TNFRSF8 levels can provide valuable insights into these diseases and potentially guide the development of targeted therapies.Overall, the Human TNFRSF8 ELISA Kit is a valuable tool for researchers studying the role of TNFRSF8 in health and disease, offering accurate and precise measurements to advance scientific understanding and therapeutic approaches.
Product Name:
Human Tumor Necrosis Factor Receptor Superfamily, Member 8 (TNFRSF8) ELISA Kit
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-101
91
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
