Human CD3d (Cluster of Differentiation 3d) CLIA Kit
The Human CD3D (Cluster of Differentiation 3D) CLIA Kit is a reliable and accurate tool for the quantification of CD3D levels in human samples such as serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit ensures precise and reproducible results, making it an ideal choice for various research applications.CD3D is a critical component of the CD3 complex, which plays a crucial role in T-cell activation and function.
Dysregulation of CD3D expression has been linked to various autoimmune diseases, immunodeficiencies, and cancer, making it a valuable biomarker for studying these conditions and potentially developing targeted therapies.Overall, the Human CD3D CLIA Kit from Assay Genie provides researchers with a powerful tool to investigate the role of CD3D in health and disease, offering valuable insights into immune responses and therapeutic strategies.
Product Name:
Human CD3d (Cluster of Differentiation 3d) CLIA Kit
SKU:
HUES00217
Target:
Human CD3d (Cluster of Differentiation 3d)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human CD3d. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human CD3d and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human CD3d, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human CD3d. You can calculate the concentration of Human CD3d in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
103-116
90-104
83-96
Average (%)
109
97
90
1:4
Range (%)
95-109
96-113
90-106
Average (%)
103
104
97
1:8
Range (%)
98-110
95-108
95-108
Average (%)
104
101
100
1:16
Range (%)
91-106
96-108
90-104
Average (%)
98
102
97
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
96-108
102
EDTA plasma (n=5)
91-104
97
Cell culture media (n=5)
86-101
93
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
110.37
290.59
717.05
101.56
313.97
717.01
Standard deviation
12.15
30.98
57.87
10.7
37.05
58.87
C V (%)
11.01
10.66
8.07
10.54
11.8
8.21
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human CD3d concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human CD3d in samples. No significant cross-reactivity or interference between Human CD3d and analogues was observed.
