Human COX5B Recombinant Protein (RPPB3237)
- SKU:
- RPPB3237
- Product Type:
- Recombinant Protein
- Species:
- Human
- Uniprot:
- P10606
Description
| Product Name: | Human COX5B Recombinant Protein |
| Product Code: | RPPB3237 |
| Size: | 10µg |
| Species: | Human |
| Target: | COX5B |
| Synonyms: | Cytochrome c oxidase subunit 5B, mitochondrial precursor, COXVB, COX5B, Mitochondrial, Cytochrome c oxidase polypeptide Vb. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile filtered colorless solution. |
| Formulation: | COX5B protein solution (0.5mg/ml) containing 20mM Tris-HCl buffer (pH8.0), 0.1M NaCl, 20% glycerol and 1mM DTT. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Purity: | Greater than 95.0% as determined by SDS-PAGE. |
| Amino Acid Sequence: | MGSSHHHHHH SSGLVPRGSH MGSASGGGVP TDEEQATGLE REIMLAAKKG LDPYNVLAPK GASGTREDPN LVPSISNKRI VGCICEEDNT SVVWFWLHKG EAQRCPRCGA HYKLVPQQLA H |
Cytochrome c oxidase subunit VB (COX5B) is the terminal enzyme of the mitochondrial respiratory chain. COX5B is a multi-subunit enzyme complex which couples the transfer of electrons from cytochrome c to molecular oxygen and contributes to a proton electrochemical gradient across the inner mitochondrial membrane. There are two isoforms of COX5:COX5a and COX5b. Transcription of COX5A (the aerobic isoform) is up-regulated as the rate of cellular respiration increases, when oxygen levels within the cell are high. However, when oxygen levels are low, COX5B (the hypoxic isoform) transcription increases and functions to maximize the turnover rate of the COX apoenzyme.
COX5B Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 121 amino acids (32-129a.a) and having a molecular mass of 13kDa. COX5B is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
| UniProt Protein Function: | COX5B: This protein is one of the nuclear-coded polypeptide chains of cytochrome c oxidase, the terminal oxidase in mitochondrial electron transport. Belongs to the cytochrome c oxidase subunit 5B family. |
| UniProt Protein Details: | Protein type:Energy Metabolism - oxidative phosphorylation; EC 1.9.3.1; Oxidoreductase; Mitochondrial Chromosomal Location of Human Ortholog: 2q11.2 Cellular Component: mitochondrion; mitochondrial inner membrane Molecular Function:cytochrome-c oxidase activity; metal ion binding Biological Process: cellular metabolic process; respiratory gaseous exchange; transmembrane transport |
| NCBI Summary: | Cytochrome C oxidase (COX) is the terminal enzyme of the mitochondrial respiratory chain. It is a multi-subunit enzyme complex that couples the transfer of electrons from cytochrome c to molecular oxygen and contributes to a proton electrochemical gradient across the inner mitochondrial membrane. The complex consists of 13 mitochondrial- and nuclear-encoded subunits. The mitochondrially-encoded subunits perform the electron transfer and proton pumping activities. The functions of the nuclear-encoded subunits are unknown but they may play a role in the regulation and assembly of the complex. This gene encodes the nuclear-encoded subunit Vb of the human mitochondrial respiratory chain enzyme. [provided by RefSeq, Jul 2008] |
| UniProt Code: | P10606 |
| NCBI GenInfo Identifier: | 20141332 |
| NCBI Gene ID: | 1329 |
| NCBI Accession: | P10606.2 |
| UniProt Related Accession: | P10606 |
| Molecular Weight: | 14kDa |
| NCBI Full Name: | Cytochrome c oxidase subunit 5B, mitochondrial |
| NCBI Synonym Full Names: | cytochrome c oxidase subunit 5B |
| NCBI Official Symbol: | COX5B�� |
| NCBI Official Synonym Symbols: | COXVB�� |
| NCBI Protein Information: | cytochrome c oxidase subunit 5B, mitochondrial |
| UniProt Protein Name: | Cytochrome c oxidase subunit 5B, mitochondrial |
| UniProt Synonym Protein Names: | Cytochrome c oxidase polypeptide Vb |
| Protein Family: | Cytochrome c oxidase polypeptide |
| UniProt Gene Name: | COX5B�� |
| UniProt Entry Name: | COX5B_HUMAN |
