The Ceruloplasmin (CP) ELISA Kit is meticulously developed for the precise and quantitative determination of ceruloplasmin levels in human samples. Ceruloplasmin, a critical copper-binding protein, serves vital roles in iron metabolism and antioxidant defense mechanisms. Monitoring ceruloplasmin levels is crucial for assessing iron homeostasis, copper transport, and overall oxidative balance in the body. Accurate measurement of ceruloplasmin helps in understanding its implications in various metabolic processes and clinical conditions. Our CP ELISA Kit offers exceptional sensitivity and specificity, ensuring accurate and reproducible results for the quantification of ceruloplasmin in diverse biological samples. With stringent quality control measures in place, this kit guarantees robust performance and straightforward protocols, making it an optimal choice for both research and clinical applications. Rely on Assay Genie's CP ELISA Kit for reliable and precise quantification of this essential biomarker, facilitating insightful studies in your research endeavors.
Product Name:
Human CP (Ceruloplasmin) ELISA Kit
SKU:
AEES00062
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
0.47 μg/mL
Detection range:
0.78-50 μg/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
