Human CTLA4 (Cytotoxic T Lymphocyte Associated Antigen 4) CLIA Kit
The Human CTLA4 (Cytotoxic T Lymphocyte-Associated Antigen 4) CLIA Kit is a cutting-edge tool designed for the precise measurement of CTLA4 levels in human samples such as serum, plasma, and cell culture supernatants. With its exceptional sensitivity and specificity, this kit ensures the accuracy and consistency of results, catering to a diverse range of research needs.CTLA4 is a key immune checkpoint protein that regulates T cell activation and plays a crucial role in maintaining immune homeostasis.
Dysregulation of CTLA4 has been implicated in various autoimmune diseases, cancer, and infectious diseases, making it a valuable biomarker for understanding disease mechanisms and developing targeted therapies.The Human CTLA4 CLIA Kit from AssayGenie provides researchers with a reliable and efficient tool to explore the intricate role of CTLA4 in health and disease, paving the way for advancements in personalized medicine and therapeutic interventions.
Product Name:
Human CTLA4 (Cytotoxic T Lymphocyte Associated Antigen 4) CLIA Kit
SKU:
HUES01102
Target:
Human CTLA4 (Cytotoxic T Lymphocyte Associated Antigen 4)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human CTLA4. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human CTLA4 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human CTLA4, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human CTLA4. You can calculate the concentration of Human CTLA4 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
91-103
88-100
92-105
Average (%)
97
93
97
1:4
Range (%)
86-100
93-106
96-110
Average (%)
93
99
102
1:8
Range (%)
97-114
101-114
97-111
Average (%)
105
107
105
1:16
Range (%)
87-99
103-116
104-119
Average (%)
94
110
110
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
87-101
94
EDTA plasma (n=5)
99-112
106
Cell culture media (n=5)
92-107
99
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
103.85
204.77
783.25
108.3
216.35
803.55
Standard deviation
9.73
17.73
51.62
10.95
24.47
68.94
C V (%)
9.37
8.66
6.59
10.11
11.31
8.58
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human CTLA4 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human CTLA4 in samples. No significant cross-reactivity or interference between Human CTLA4 and analogues was observed.
