The Human DEFA5 (Defensin Alpha 5) Paneth Cell-Specific CLIA Kit is specifically designed for the precise measurement of DEFA5 levels in human samples such as serum, plasma, and cell culture supernatants. This cutting-edge kit boasts exceptional sensitivity and specificity, ensuring accurate and reproducible results for a variety of research applications.Defensin Alpha 5 is a noteworthy protein that plays a critical role in the immune system by combating microbial infections and maintaining gut homeostasis.
It is predominantly expressed in Paneth cells of the small intestine and has been implicated in various inflammatory and infectious diseases. Therefore, the DEFA5 CLIA Kit serves as a valuable tool for investigating the role of DEFA5 in health and disease, as well as for potential therapeutic interventions in related conditions.
Human DEF alpha5 (Defensin Alpha 5, Paneth Cell Specific)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
4.69 pg/mL
Detection range:
7.81-500 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human DEFα5. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human DEFα5 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human DEFα5, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human DEFα5. You can calculate the concentration of Human DEFα5 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
93-107
87-99
93-106
Average (%)
99
93
99
1:4
Range (%)
102-114
93-111
87-100
Average (%)
108
101
93
1:8
Range (%)
88-100
102-120
90-105
Average (%)
93
110
97
1:16
Range (%)
94-104
99-112
97-110
Average (%)
99
105
104
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
90-101
95
EDTA plasma (n=5)
96-110
103
Cell culture media (n=5)
88-101
93
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
26.82
75.83
186.53
26.3
73.22
184.8
Standard deviation
3.19
7.33
11.21
3.05
8.27
15.15
C V (%)
11.89
9.67
6.01
11.6
11.29
8.2
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human DEFα5 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human DEFα5 in samples. No significant cross-reactivity or interference between Human DEFα5 and analogues was observed.
