Human ENO3 Recombinant Protein (RPPB1617)
- SKU:
- RPPB1617
- Product Type:
- Recombinant Protein
- Species:
- Human
- Uniprot:
- P13929
- Research Area:
- Enzymes
Description
| Product Name: | Human ENO3 Recombinant Protein |
| Product Code: | RPPB1617 |
| Size: | 10µg |
| Species: | Human |
| Target: | ENO3 |
| Synonyms: | Enolase 3 (beta, muscle), Muscle-specific enolase, Skeletal muscle enolase, MSE, 2-phospho-D-glycerate hydrolyase, beta-enolase, GSD13, EC 4.2.1.11, EC 4.2.1. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile Filtered colorless solution. |
| Formulation: | The ENO3 solution (0.5mg/ml) contains 20mM Tris-HCl buffer (pH 8.0), 1mM DTT, 0.1M NaCl and 20% glycerol. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Purity: | Greater than 95% as determined by SDS-PAGE. |
| Amino Acid Sequence: | MGSSHHHHHH SSGLVPRGSH MAMQKIFARE ILDSRGNPTV EVDLHTAKGR FRAAVPSGAS TGIYEALELR DGDKGRYLGK GVLKAVENIN STLGPALLQK KLSVADQEKV DKFMIELDGT ENKSKFGANA ILGVSLAVCK AGAAEKGVPL YRHIADLAGN PDLILPVPAF NVINGGSHAG NKLAMQEFMI LPVGASSFKE AMRIGAEVYH HLKGVIKAKY GKDATNVGDE GGFAPNILEN NEALELLKTA IQAAGYPDKV VIGMDVAASE FYRNGKYDLD FKSPDDPARH ITGEKLGELY KSFIKNYPVV SIEDPFDQDD WATWTSFLSG VNIQIVGDDL TVTNPKRIAQ AVEKKACNCL LLKVNQIGSV TESIQACKLA QSNGWGVMVS HRSGETEDTF IADLVVGLCT GQIKTGAPCR SERLAKYNQL MRIEEALGDK AIFAGRKFRN PKAK |
ENO3 is one of three enolase isoenzymes in mammals. The homodimer ENO3 is located in skeletal muscle cells of adults and has a part in converting phosphoglyceric acid to phosphenolpyruvic acid in the glycolytic pathway. Mutations in ENO3 gene is linked to metabolic myopathies which is caused by low stability of the enzyme.
ENO3 Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 454 amino acids (1-434) and having a molecular mass of 49.0 kDa.ENO3 is fused to a 20 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
| UniProt Protein Function: | ENO3: Appears to have a function in striated muscle development and regeneration. Defects in ENO3 are the cause of glycogen storage disease type 13 (GSD13). A metabolic disorder that results in exercise-induced myalgias, generalized muscle weakness and fatigability. It is characterized by increased serum creatine kinase and decreased enolase 3 activity. Dramatically reduced protein levels with focal sarcoplasmic accumulation of glycogen- beta particles are detected on ultrastructural analysis. Belongs to the enolase family. 3 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Lyase; Carbohydrate Metabolism - glycolysis and gluconeogenesis; EC 4.2.1.11 Chromosomal Location of Human Ortholog: 17p13.2 Cellular Component: extracellular space; phosphopyruvate hydratase complex; cytoplasm; plasma membrane; cytosol Molecular Function:protein homodimerization activity; protein heterodimerization activity; magnesium ion binding; phosphopyruvate hydratase activity Biological Process: response to drug; glycolysis; carbohydrate metabolic process; skeletal muscle regeneration; glucose metabolic process; pathogenesis; gluconeogenesis; aging Disease: Glycogen Storage Disease Xiii |
| NCBI Summary: | This gene encodes one of the three enolase isoenzymes found in mammals. This isoenzyme is found in skeletal muscle cells in the adult where it may play a role in muscle development and regeneration. A switch from alpha enolase to beta enolase occurs in muscle tissue during development in rodents. Mutations in this gene have be associated glycogen storage disease. Alternatively spliced transcript variants encoding different isoforms have been described.[provided by RefSeq, Jul 2010] |
| UniProt Code: | P13929 |
| NCBI GenInfo Identifier: | 425906077 |
| NCBI Gene ID: | 2027 |
| NCBI Accession: | P13929.5 |
| UniProt Related Accession: | P13929 |
| Molecular Weight: | |
| NCBI Full Name: | Beta-enolase |
| NCBI Synonym Full Names: | enolase 3 |
| NCBI Official Symbol: | ENO3�� |
| NCBI Official Synonym Symbols: | MSE; GSD13�� |
| NCBI Protein Information: | beta-enolase |
| UniProt Protein Name: | Beta-enolase |
| UniProt Synonym Protein Names: | 2-phospho-D-glycerate hydro-lyase; Enolase 3; Muscle-specific enolase; MSE; Skeletal muscle enolase |
| Protein Family: | Enolase |
| UniProt Gene Name: | ENO3�� |
| UniProt Entry Name: | ENOB_HUMAN |
