The Human F11 (Coagulation Factor XI) CLIA Kit is a highly accurate and reliable assay designed for the quantitative measurement of Coagulation Factor XI levels in human serum, plasma, and cell culture supernatants. This kit provides exceptional sensitivity and specificity, ensuring precise and reproducible results for a variety of research applications.Coagulation Factor XI is a critical protein that plays a key role in the blood coagulation cascade, contributing to the formation of blood clots.
Dysregulation of Factor XI levels has been implicated in various coagulation disorders, making it a valuable biomarker for studying these conditions and developing potential therapeutic interventions.Overall, the Human F11 (Coagulation Factor XI) CLIA Kit offers researchers a powerful tool for investigating the role of Factor XI in coagulation disorders and advancing the field of coagulation research.
Product Name:
Human F11 (Coagulation Factor XI) CLIA Kit
SKU:
HUES00480
Target:
Human F11 (Coagulation Factor XI)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
37.50 pg/mL
Detection range:
62.50-4000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human F11. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human F11 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human F11, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human F11. You can calculate the concentration of Human F11 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
103-115
90-103
92-103
Average (%)
108
95
98
1:4
Range (%)
86-99
92-109
89-102
Average (%)
92
100
95
1:8
Range (%)
93-108
88-103
99-113
Average (%)
101
94
105
1:16
Range (%)
98-116
89-102
85-99
Average (%)
106
97
91
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
84-97
90
EDTA plasma (n=5)
93-107
98
Cell culture media (n=5)
87-99
93
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
201.15
543.25
1689.62
187.99
562.95
1616.64
Standard deviation
24.64
59.76
179.1
19.95
59.56
123.67
C V (%)
12.25
11.0
10.6
10.61
10.58
7.65
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human F11 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human F11 in samples. No significant cross-reactivity or interference between Human F11 and analogues was observed.
