Human FARS2 Recombinant Protein (RPPB1630)
- SKU:
- RPPB1630
- Product Type:
- Recombinant Protein
- Species:
- Human
- Uniprot:
- O95363
- Research Area:
- Enzymes
Description
| Product Name: | Human FARS2 Recombinant Protein |
| Product Code: | RPPB1630 |
| Size: | 10µg |
| Species: | Human |
| Target: | FARS2 |
| Synonyms: | Phenylalanyl-tRNA synthetase mitochondrial, Phenylalanine--tRNA ligase, PheRS, FARS2, FARS1, HSPC320, dJ520B18.2. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile filtered colorless solution. |
| Formulation: | FARS2 solution (0.5mg/ml) containing 20mM Tris-HCl buffer (pH 8.0), 1mM DTT, 30% glycerol, 1mM EDTA and 0.1M NaCl. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Purity: | Greater than 90.0% as determined by SDS-PAGE. |
| Amino Acid Sequence: | MGSSHHHHHH SSGLVPRGSH MPAAECATQR APGSVVELLG KSYPQDDHSN LTRKVLTRVG RNLHNQQHHP LWLIKERVKE HFYKQYVGRF GTPLFSVYDN LSPVVTTWQN FDSLLIPADH PSRKKGDNYY LNRTHMLRAH TSAHQWDLLH AGLDAFLVVG DVYRRDQIDS QHYPIFHQLE AVRLFSKHEL FAGIKDGESL QLFEQSSRSA HKQETHTMEA VKLVEFDLKQ TLTRLMAHLF GDELEIRWVD CYFPFTHPSF EMEINFHGEW LEVLGCGVME QQLVNSAGAQ DRIGWAFGLG LERLAMILYD IPDIRLFWCE DERFLKQFCV SNINQKVKFQ PLSKYPAVIN DISFWLPSEN YAENDFYDLV RTIGGDLVEK VDLIDKFVHP KTHKTSHCYR ITYRHMERTL SQREVRHIHQ ALQEAAVQLL GVEGRF |
Phenylalanyl-tRNA synthetase 2 mitochondrial (FARS2) is a member of the class II aminoacyl- tRNA synthetase family. FARS2 is a mitochondrial matrix protein. FARS2 when acting as a monomer, catalyzes the ATP-dependent conversion of L-phenylalanine and tRNA (Phe) to L-phenlalanyltRNA (Phe), an event which is vital for proper translation and protein expression.
FARS2 produced in E.Coli is a single, non-glycosylated polypeptide chain containing 436 amino acids (37-451 a.a.) and having a molecular mass of 50.6kDa.FARS2 is fused to a 21 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
| UniProt Protein Function: | FARS2: Catalyzes direct attachment of p-Tyr (Tyr) to tRNAPhe. Permits also, with a lower efficiency, the attachment of m-Tyr to tRNAPhe, thereby opening the way for delivery of the misacylated tRNA to the ribosome and incorporation of ROS-damaged amino acid into proteins. Belongs to the class-II aminoacyl-tRNA synthetase family. |
| UniProt Protein Details: | Protein type:EC 6.1.1.20; RNA-binding; Translation; Mitochondrial; Ligase Chromosomal Location of Human Ortholog: 6p25.1 Cellular Component: mitochondrial matrix Molecular Function:phenylalanine-tRNA ligase activity; magnesium ion binding; ATP binding; tRNA binding Biological Process: tRNA aminoacylation for protein translation; tRNA processing; phenylalanyl-tRNA aminoacylation; gene expression Disease: Combined Oxidative Phosphorylation Deficiency 14 |
| UniProt Code: | O95363 |
| NCBI GenInfo Identifier: | 15341941 |
| NCBI Gene ID: | 64108 |
| NCBI Accession: | BC013161 |
| UniProt Secondary Accession: | O95363,Q9H4F3, |
| UniProt Related Accession: | Q96DX8 |
| Molecular Weight: | 27,863 Da |
| NCBI Full Name: | Homo sapiens receptor (chemosensory) transporter protein 4, mRNA |
| NCBI Synonym Full Names: | receptor transporter protein 4 |
| NCBI Official Symbol: | RTP4�� |
| NCBI Official Synonym Symbols: | IFRG28; Z3CXXC4�� |
| NCBI Protein Information: | receptor-transporting protein 4 |
| UniProt Protein Name: | Phenylalanine--tRNA ligase, mitochondrial |
| UniProt Synonym Protein Names: | Phenylalanyl-tRNA synthetase; PheRS |
| Protein Family: | Receptor-transporting protein |
| UniProt Gene Name: | FARS2�� |
| UniProt Entry Name: | SYFM_HUMAN |
