The Human Galectin-9 (Gal9) ELISA Kit from AssayGenie is a powerful tool for the accurate measurement of Galectin-9 levels in human samples. Galectin-9 is a multifunctional protein that plays a crucial role in various biological processes, including immune regulation, inflammation, and cancer progression.This ELISA kit offers high sensitivity and specificity, ensuring precise and reliable results for researchers studying Galectin-9 in serum, plasma, and cell culture supernatants. By accurately measuring Galectin-9 levels, researchers can gain valuable insights into its role in various diseases and potential therapeutic applications.
Galectin-9 has been implicated in a wide range of conditions, including autoimmune disorders, infectious diseases, and cancer. By using the Human Galectin-9 ELISA Kit, researchers can better understand the mechanisms underlying these diseases and identify new targets for intervention.Overall, the Human Galectin-9 ELISA Kit from AssayGenie is a valuable tool for researchers looking to investigate the role of Galectin-9 in health and disease. With its high sensitivity and specificity, this kit provides reliable and reproducible results, making it an essential addition to any research laboratory.
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
