Human GLRX5 Recombinant Protein (RPPB1704)
- SKU:
- RPPB1704
- Product Type:
- Recombinant Protein
- Species:
- Human
- Uniprot:
- Q86SX6
- Research Area:
- Enzymes
Description
| Product Name: | Human GLRX5 Recombinant Protein |
| Product Code: | RPPB1704 |
| Size: | 25µg |
| Species: | Human |
| Target: | GLRX5 |
| Synonyms: | Glutaredoxin-related protein 5 mitochondrial, Monothiol glutaredoxin-5, GLRX5, C14orf87, glutaredoxin 5, GRX5, FLB4739, PR01238, PRO1238, MGC14129. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile filtered colorless solution. |
| Formulation: | GLRX5 solution containing 20mM Tris-HCl buffer (pH8.0), 20% glycerol and 0.1M NaCl. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Purity: | Greater than 85% as determined by SDS-PAGE. |
| Amino Acid Sequence: | MGSSHHHHHH SSGLVPRGSH MSGSLGRAAA ALLRWGRGAG GGGLWGPGVR AAGSGAGGGG SAEQLDALVK KDKVVVFLKG TPEQPQCGFS NAVVQILRLH GVRDYAAYNV LDDPELRQGI KDYSNWPTIP QVYLNGEFVG GCDILLQMHQ NGDLVEELKK LGIHSALLDE KKDQDSK |
GLRX5 is small redox enzyme of approximately 100 amino acids which uses glutathione as a cofactor. GLRX5 is a mitochondrial protein, which is evolutionarily conserved. GLRX5 is oxidized by substrates, and reduced non-enzymatically by glutathione. GLRX5 is involved in the biogenesis of iron-sulfur clusters that are required for normal iron homeostasis. GLRX5 is necessary for normal regulation of hemoglobin synthesis by the iron-sulfur protein ACO1. Defects in the GLRX5 gene are a cause of anemia sideroblastic pyridoxine-refractory autosomal recessive (PRARSA).
GLRX5 Human Recombinant produced in E.Coli is a single, non-glycosylated,polypeptide chain containing 177 amino acids (1-157 a.a.) and having a molecular mass of 18.8 kDa. GRX5 is fused to a 20 amino acid His Tag at N-terminus and purified by proprietary chromatographic techniques.
| UniProt Protein Function: | GLRX5: Monothiol glutaredoxin involved in the biogenesis of iron-sulfur clusters. Required for normal iron homeostasis. Required for normal regulation of hemoglobin synthesis by the iron-sulfur protein ACO1. Defects in GLRX5 are a cause of anemia sideroblastic pyridoxine-refractory autosomal recessive (PRARSA). A form of sideroblastic anemia not responsive to pyridoxine. Sideroblastic anemia is characterized by anemia of varying severity, hypochromic peripheral erythrocytes, systemic iron overload secondary to chronic ineffective erythropoiesis, and the presence of bone marrow ringed sideroblasts. Sideroblasts are characterized by iron-loaded mitochondria clustered around the nucleus. Belongs to the glutaredoxin family. Monothiol subfamily.Protein type: OxidoreductaseChromosomal Location of Human Ortholog: 14q32.13Cellular Component: mitochondrial matrix; mitochondrionBiological Process: hemopoiesisDisease: Anemia, Sideroblastic, Pyridoxine-refractory, Autosomal Recessive |
| UniProt Protein Details: | |
| NCBI Summary: | This gene encodes a mitochondrial protein, which is evolutionarily conserved. It is involved in the biogenesis of iron-sulfur clusters, which are required for normal iron homeostasis. Mutations in this gene are associated with autosomal recessive pyridoxine-refractory sideroblastic anemia. [provided by RefSeq, May 2010] |
| UniProt Code: | Q86SX6 |
| NCBI GenInfo Identifier: | 83288163 |
| NCBI Gene ID: | 51218 |
| NCBI Accession: | Q86SX6.2 |
| UniProt Secondary Accession: | Q86SX6,Q0X088, Q3YML0, Q86WY3, Q8IZ54 |
| UniProt Related Accession: | Q86SX6 |
| Molecular Weight: | 16,628 Da |
| NCBI Full Name: | Glutaredoxin-related protein 5, mitochondrial |
| NCBI Synonym Full Names: | glutaredoxin 5 |
| NCBI Official Symbol: | GLRX5�� |
| NCBI Official Synonym Symbols: | GRX5; PRSA; SIDBA3; SPAHGC; FLB4739; PR01238; PRO1238; C14orf87�� |
| NCBI Protein Information: | glutaredoxin-related protein 5, mitochondrial |
| UniProt Protein Name: | Glutaredoxin-related protein 5, mitochondrial |
| UniProt Synonym Protein Names: | Monothiol glutaredoxin-5 |
| Protein Family: | Glutaredoxin-related protein |
| UniProt Gene Name: | GLRX5�� |
| UniProt Entry Name: | GLRX5_HUMAN |
