Human GPT2 Recombinant Protein (RPPB1740)
- SKU:
- RPPB1740
- Product Type:
- Recombinant Protein
- Species:
- Human
- Uniprot:
- Q8TD30
- Research Area:
- Enzymes
Description
| Product Name: | Human GPT2 Recombinant Protein |
| Product Code: | RPPB1740 |
| Size: | 10µg |
| Species: | Human |
| Target: | GPT2 |
| Synonyms: | ALT2, AAT2, Alanine aminotransferase 2, Glutamate pyruvate transaminase 2, Glutamic--alanine transaminase 2, Glutamic--pyruvic transaminase 2. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile Filtered colorless solution. |
| Formulation: | The GPT2 solution (0.5mg/1ml) contains 20mM Tris-HCl buffer (pH7.5), 30% glycerol, 2mM DTT, 0.2M NaCl. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Purity: | Greater than 90% as determined by SDS-PAGE. |
| Amino Acid Sequence: | MGSSHHHHHHSSGLVPRGSH MGSMQRAAALVRRGCGPRTP SSWGRSQSSA AAEASAVLKV RPERSRRERI LTLESMNPQV KAVEYAVRGP IVLKAGEIELELQRGIKKPF TEVIRANIGD AQAMGQQPIT FLRQVMALCT YPNLLDSPSF PEDAKKRARR ILQACGGNSLGSYSASQGVN CIREDVAAYI TRRDGGVPAD PDNIYLTTGA SDGISTILKI LVSGGGKSRT GVMIPIPQYPLYSAVISELD AIQVNYYLDE ENCWALNVNE LRRAVQEAKD HCDPKVLCII NPGNPTGQVQ SRKCIEDVIHFAWEEKLFLL ADEVYQDNVY SPDCRFHSFK KVLYEMGPEY SSNVELASFH STSKGYMGEC GYRGGYMEVINLHPEIKGQL VKLLSVRLCP PVSGQAAMDI VVNPPVAGEE SFEQFSREKE SVLGNLAKKA KLTEDLFNQV PGIHCNPLQGAMYAFPRIFI PAKAVEAAQA HQMAPDMFYC MKLLEETGIC VVPGSGFGQR EGTYHFRMTI LPPVEKLKTVLQKVKDFHIN FLEKYA |
| Biological Activity: | Specific activity is > 100units/mg, and is defined asthe amount of enzyme that cleaves 1umole of L-Alanine to L-Glutamate per minuteat pH 7.5 at 37C. |
Alanine aminotransferase 2 (GPT2), catalyzes the reversible transamination among alanine and 2-oxoglutarate to create pyruvate and glutamate. GPT2 expressed mainly in muscle, fat and kidney and participates in the intermediary metabolism of glucose and amino acids. Multiple transcript variants encoding various isoforms have been found for GPT2.
GPT2 Human Recombinant produced in E. coli is a single polypeptide chain containing 546 amino acids (1-523) and having a molecular mass of 60.3 kDa. GPT2 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
| UniProt Protein Function: | GPT2: Catalyzes the reversible transamination between alanine and 2-oxoglutarate to form pyruvate and glutamate. Belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family. Alanine aminotransferase subfamily. 2 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:EC 2.6.1.2; Amino Acid Metabolism - alanine, aspartate and glutamate; Transferase; Mitochondrial Chromosomal Location of Human Ortholog: 16q12.1 Cellular Component: mitochondrial matrix Molecular Function:alanine transaminase activity; pyridoxal phosphate binding Biological Process: 2-oxoglutarate metabolic process; amino acid biosynthetic process; L-alanine catabolic process; L-alanine metabolic process Disease: Mental Retardation, Autosomal Recessive 49 |
| NCBI Summary: | This gene encodes a mitochondrial alanine transaminase, a pyridoxal enzyme that catalyzes the reversible transamination between alanine and 2-oxoglutarate to generate pyruvate and glutamate. Alanine transaminases play roles in gluconeogenesis and amino acid metabolism in many tissues including skeletal muscle, kidney, and liver. Activating transcription factor 4 upregulates this gene under metabolic stress conditions in hepatocyte cell lines. A loss of function mutation in this gene has been associated with developmental encephalopathy. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Apr 2015] |
| UniProt Code: | Q8TD30 |
| NCBI GenInfo Identifier: | 74730602 |
| NCBI Gene ID: | 84706 |
| NCBI Accession: | Q8TD30.1 |
| UniProt Secondary Accession: | Q8TD30,Q8N9E2, |
| UniProt Related Accession: | Q8TD30 |
| Molecular Weight: | 46,983 Da |
| NCBI Full Name: | Alanine aminotransferase 2 |
| NCBI Synonym Full Names: | glutamic pyruvate transaminase (alanine aminotransferase) 2 |
| NCBI Official Symbol: | GPT2�� |
| NCBI Official Synonym Symbols: | ALT2; GPT 2; MRT49�� |
| NCBI Protein Information: | alanine aminotransferase 2 |
| UniProt Protein Name: | Alanine aminotransferase 2 |
| UniProt Synonym Protein Names: | Glutamate pyruvate transaminase 2; GPT 2; Glutamic--alanine transaminase 2; Glutamic--pyruvic transaminase 2 |
| Protein Family: | Xanthine phosphoribosyltransferase |
| UniProt Gene Name: | GPT2�� |
| UniProt Entry Name: | ALAT2_HUMAN |
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