The Human High-Sensitive CRP ELISA Kit is a powerful tool for measuring C-reactive protein (CRP) levels in human samples such as serum and plasma with high sensitivity and accuracy. CRP is a key marker of inflammation and is commonly used in clinical settings to assess and monitor various inflammatory conditions.This ELISA kit is designed to provide precise and reproducible results, making it suitable for a wide range of research applications including studies on cardiovascular diseases, infections, autoimmune disorders, and more. With its reliable performance and easy-to-use format, the Human High-Sensitive CRP ELISA Kit is an essential tool for researchers and healthcare professionals alike.
Product Name:
Human CRP ELISA Kit (High Sensitivity)
SKU:
HUES03393
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
