Human IL 3 Recombinant Protein (RPPB0495)
- SKU:
- RPPB0495
- Product Type:
- Recombinant Protein
- Species:
- Human
- Uniprot:
- P08700
- Research Area:
- Cytokines
Description
| Product Name: | Human IL 3 Recombinant Protein |
| Product Code: | RPPB0495 |
| Size: | 50µg |
| Species: | Human |
| Target: | IL 3 |
| Synonyms: | MCGF (Mast cell growth factor), Multi-CSF, HCGF, P-cell stimulation factor, IL-3, MGC79398, MGC79399. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile Filtered clear solution. |
| Formulation: | Interleukin-3 His (1mg/ml) is supplied in 20mM Tris-HCl buffer (pH 8.0), 0.2mM PMSF and 10% glycerol. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Purity: | Greater than 90.0% as determined by analysis by SDS-PAGE. |
| Amino Acid Sequence: | MGSSHHHHHH SSGLVPRGSH MAPMTQTTSL KTSWVNCSNM IDEIITHLKQ PPLPLLDFNN LNGEDQDILM ENNLRRPNLE AFNRAVKSLQ NASAIESILK NLLPCLPLAT AAPTRHPIHI KDGDWNEFRR KLTFYLKTLE NAQAQQTTLS LAIF |
| Biological Activity: | The ED50 for this effect is <0.53ng/ml. Measured in a cell proliferation assay using TF1 human erythroleukemic cells. |
Interleukin-3 is a pleiotropic cytokine produced primarily by activated T cells. IL-3 is thought to function via specific cell surface receptors to stimulate the proliferation, differentiation and survival of haematopoietic cell lines. IL-3 has also been shown to affect the functional activity of a variety of other cell types including mast cells, eosinophils, megakaryocytes and basophils.
Interleukin-3 Human Recombinant produced in E.Coli is single, a non-glycosylated, Polypeptide chain containing 154 amino acids fragment (20-152) and having a total molecular mass of 17.3kDa and fused with a 20 aa N-terminal His tag. The IL3 His is purified by proprietary chromatographic techniques.
| UniProt Protein Function: | IL3: Granulocyte/macrophage colony-stimulating factors are cytokines that act in hematopoiesis by controlling the production, differentiation, and function of 2 related white cell populations of the blood, the granulocytes and the monocytes-macrophages. Belongs to the IL-3 family. |
| UniProt Protein Details: | Protein type:Cell cycle regulation; Secreted, signal peptide; Secreted; Cytokine; Oncoprotein; Apoptosis Chromosomal Location of Human Ortholog: 5q31.1 Cellular Component: extracellular space; extracellular region Molecular Function:interleukin-3 receptor binding; growth factor activity; cytokine activity Biological Process: nervous system development; positive regulation of peptidyl-tyrosine phosphorylation; cell-cell signaling; positive regulation of tyrosine phosphorylation of Stat5 protein; embryonic hemopoiesis; cytokine and chemokine mediated signaling pathway; positive regulation of cell proliferation; immune response; positive regulation of myeloid leukocyte differentiation; positive regulation of DNA replication |
| NCBI Summary: | The protein encoded by this gene is a potent growth promoting cytokine. This cytokine is capable of supporting the proliferation of a broad range of hematopoietic cell types. It is involved in a variety of cell activities such as cell growth, differentiation and apoptosis. This cytokine has been shown to also possess neurotrophic activity, and it may be associated with neurologic disorders. [provided by RefSeq, Jul 2008] |
| UniProt Code: | P08700 |
| NCBI GenInfo Identifier: | 124330 |
| NCBI Gene ID: | 3562 |
| NCBI Accession: | P08700.2 |
| UniProt Secondary Accession: | P08700,Q6GS87, |
| UniProt Related Accession: | P08700 |
| Molecular Weight: | 17,233 Da |
| NCBI Full Name: | Interleukin-3 |
| NCBI Synonym Full Names: | interleukin 3 |
| NCBI Official Symbol: | IL3�� |
| NCBI Official Synonym Symbols: | IL-3; MCGF; MULTI-CSF�� |
| NCBI Protein Information: | interleukin-3; mast cell growth factor; mast-cell growth factor; P-cell stimulating factor; P-cell-stimulating factor; hematopoietic growth factor; colony-stimulating factor, multiple; multilineage-colony-stimulating factor; multipotential colony-stimulating factor |
| UniProt Protein Name: | Interleukin-3 |
| UniProt Synonym Protein Names: | Hematopoietic growth factor; Mast cell growth factor; MCGF; Multipotential colony-stimulating factor; P-cell-stimulating factor |
| Protein Family: | Interleukin |
| UniProt Gene Name: | IL3�� |
| UniProt Entry Name: | IL3_HUMAN |
