The Human IL-1 Alpha ELISA Kit is a powerful tool for measuring levels of interleukin-1 alpha in human samples. IL-1 alpha is a critical cytokine involved in inflammatory responses, immune regulation, and cell growth, making it essential for studying various diseases and conditions.This ELISA kit offers high sensitivity and specificity, providing accurate and reliable results for serum, plasma, and cell culture supernatants.
Researchers can trust the precision of this kit for their studies in areas such as autoimmune diseases, inflammatory disorders, and cancer research.With its user-friendly protocol and quick turnaround time, the Human IL-1 Alpha ELISA Kit from Assay Genie is an excellent choice for researchers looking to delve deeper into the role of interleukin-1 alpha in human health and disease.
Product Name:
Human IL-1 alpha ELISA Kit
SKU:
HUES01374
Target:
Human IL-1 alpha
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
2.35 pg/mL
Detection range:
3.91-250 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human IL-1α. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human IL-1α and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human IL-1α, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human IL-1α. You can calculate the concentration of Human IL-1α in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
92-107
97-111
88-101
Average (%)
100
103
93
1:4
Range (%)
94-107
83-95
95-109
Average (%)
101
89
103
1:8
Range (%)
99-113
85-99
91-103
Average (%)
104
91
98
1:16
Range (%)
94-107
82-92
94-108
Average (%)
101
87
99
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
88-102
93
EDTA plasma (n=5)
91-104
96
Cell culture media (n=5)
87-98
93
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
11.9
22.7
113.0
11.6
22.4
105.7
Standard deviation
0.7
1.2
3.6
0.8
1.3
3.7
C V (%)
5.88
5.29
3.19
6.9
5.8
3.5
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human IL-1α concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human IL-1α in samples. No significant cross-reactivity or interference between Human IL-1α and analogues was observed.
