The Human IL-10 CLIA Kit offered by AssayGenie is a highly sensitive and specific assay designed for the accurate detection of interleukin-10 (IL-10) levels in human samples, including serum, plasma, and cell culture supernatants. IL-10 is a key cytokine that plays a crucial role in regulating immune responses and inflammation.This kit provides researchers with a reliable tool for studying the role of IL-10 in various disease states, including autoimmune disorders, inflammatory conditions, and cancer. With its high sensitivity and reproducibility, the Human IL-10 CLIA Kit is an ideal choice for a wide range of research applications, offering valuable insights into the mechanisms underlying these diseases and potential targets for therapeutic intervention.
Overall, the Human IL-10 CLIA Kit from AssayGenie is a valuable resource for researchers seeking to study the role of IL-10 in human health and disease, providing accurate and reliable results that can drive advancements in the field of immunology and inflammation research.
Product Name:
Human IL-10 CLIA Kit
SKU:
HUES00099
Target:
Human IL-10
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
4.69 pg/mL
Detection range:
7.81-500 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human IL-10. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human IL-10 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human IL-10, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human IL-10. You can calculate the concentration of Human IL-10 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
91-105
99-114
83-96
Average (%)
96
105
90
1:4
Range (%)
102-119
97-113
91-106
Average (%)
109
105
98
1:8
Range (%)
87-99
99-116
94-106
Average (%)
93
106
100
1:16
Range (%)
101-117
86-98
89-103
Average (%)
108
91
96
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
92-106
98
EDTA plasma (n=5)
90-101
95
Cell culture media (n=5)
86-97
91
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
24.45
46.94
167.57
22.04
49.29
171.49
Standard deviation
3.13
5.51
16.12
2.52
5.52
13.31
C V (%)
12.8
11.74
9.62
11.43
11.2
7.76
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human IL-10 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human IL-10 in samples. No significant cross-reactivity or interference between Human IL-10 and analogues was observed.
