The Human IL-17 CLIA Kit is a cutting-edge assay designed for the precise measurement of IL-17 levels in human samples, including serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, guaranteeing accurate and consistent results for a variety of research purposes.IL-17 is a key cytokine that plays a critical role in inflammatory responses and autoimmune diseases.
By detecting IL-17 levels, researchers can gain valuable insights into the mechanisms underlying these conditions and explore potential therapeutic targets. The Human IL-17 CLIA Kit is an essential tool for studying immune-mediated diseases and developing innovative treatment strategies.
Product Name:
Human IL-17 CLIA Kit
SKU:
HUES00101
Target:
Human IL-17
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
7.50 pg/mL
Detection range:
12.50-800 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human IL-17. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human IL-17 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human IL-17, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human IL-17. You can calculate the concentration of Human IL-17 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
88-103
101-118
91-107
Average (%)
95
108
98
1:4
Range (%)
87-103
91-108
95-108
Average (%)
94
99
101
1:8
Range (%)
88-103
86-96
86-99
Average (%)
96
91
91
1:16
Range (%)
88-103
93-108
101-117
Average (%)
95
99
108
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
97-114
104
EDTA plasma (n=5)
99-114
105
Cell culture media (n=5)
89-102
95
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
42.68
66.99
285.27
44.38
65.34
282.26
Standard deviation
4.08
7.6
31.89
4.29
6.61
26.76
C V (%)
9.56
11.34
11.18
9.67
10.12
9.48
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human IL-17 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human IL-17 in samples. No significant cross-reactivity or interference between Human IL-17 and analogues was observed.
