Human IL5 Recombinant Protein (RPPB0516)
- SKU:
- RPPB0516
- Product Type:
- Recombinant Protein
- Species:
- Human
- Uniprot:
- P05113
- Research Area:
- Cytokines
Description
| Product Name: | Human IL5 Recombinant Protein |
| Product Code: | RPPB0516 |
| Size: | 10µg |
| Species: | Human |
| Target: | IL5 |
| Synonyms: | EDF, BCDFII, TRF, T-cell replacing factor, Eosinophil differentiation factor, B cell differentiation factor I, IL-5. |
| Source: | Sf9 Insect cells |
| Physical Appearance: | Sterile filtered colorless solution. |
| Formulation: | IL5 protein solution (0.5mg/ml) contains Phosphate Buffered Saline (pH 7.4) and 10% glycerol. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Purity: | Greater than 90.0% as determined by SDS-PAGE. |
| Amino Acid Sequence: | ADPIPTEIPT SALVKETLAL LSTHRTLLIA NETLRIPVPV HKNHQLCTEE IFQGIGTLES QTVQGGTVER LFKNLSLIKK YIDGQKKKCG EERRRVNQFL DYLQEFLGVM NTEWIIESHH HHHH |
| Biological Activity: | Measured in a cell proliferation assay using TF-1 human erythroleukemic cell. The ED50 for this effect is less or equal to 1.5 ng/ml. |
The protein encoded by this gene is a cytokine that acts as a growth and differentiation factor for both B cells and eosinophils. This cytokine is a main regulator of eosinopoiesis, eosinophil maturation and activation. The elevated production of this cytokine is reported to be related to asthma or hypereosinophilic syndromes. The receptor of this cytokine is a heterodimer, whose beta subunit is shared with the receptors for interleukine 3 (IL3) and colony stimulating factor 2 (CSF2/GM-CSF). This gene, together with those for interleukin 4 (IL4), interleukin 13 (IL13), and CSF2, form a cytokine gene cluster on chromosome 5. This cytokine, IL4, and IL13 are found to be regulated coordinately by long-range regulatory elements spread over 120 kilobases on chromosome 5q31.
Interleukin-5 Human Recombinant produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain containing 124 amino acids (20-134a.a.) and having a molecular mass of 14.2kDa (Molecular size on SDS-PAGE will appear at approximately 13.5-18kDa).IL5 is expressed with a 6 amino acid His-tag at C-Terminus and purified by proprietary chromatographic techniques.
| UniProt Protein Function: | IL5: Factor that induces terminal differentiation of late- developing B-cells to immunoglobulin secreting cells. Belongs to the IL-5 family. |
| UniProt Protein Details: | Protein type:Secreted; Secreted, signal peptide; Cytokine Chromosomal Location of Human Ortholog: 5q31.1 Cellular Component: extracellular region; extracellular space; intracellular Molecular Function:cytokine activity; growth factor activity; interleukin-5 receptor binding; protein binding Biological Process: activation of MAPKK activity; axon guidance; cytokine and chemokine mediated signaling pathway; epidermal growth factor receptor signaling pathway; fibroblast growth factor receptor signaling pathway; inflammatory response; innate immune response; insulin receptor signaling pathway; MAPKKK cascade; nerve growth factor receptor signaling pathway; positive regulation of B cell proliferation; positive regulation of eosinophil differentiation; positive regulation of immunoglobulin secretion; positive regulation of JAK-STAT cascade; positive regulation of peptidyl-tyrosine phosphorylation; positive regulation of transcription factor activity; Ras protein signal transduction; small GTPase mediated signal transduction; vascular endothelial growth factor receptor signaling pathway |
| NCBI Summary: | This gene encodes a cytokine that acts as a growth and differentiation factor for both B cells and eosinophils. The encoded cytokine plays a major role in the regulation of eosinophil formation, maturation, recruitment and survival. The increased production of this cytokine may be related to pathogenesis of eosinophil-dependent inflammatory diseases. This cytokine functions by binding to its receptor, which is a heterodimer, whose beta subunit is shared with the receptors for interleukine 3 (IL3) and colony stimulating factor 2 (CSF2/GM-CSF). This gene is located on chromosome 5 within a cytokine gene cluster which includes interleukin 4 (IL4), interleukin 13 (IL13), and CSF2 . This gene, IL4, and IL13 may be regulated coordinately by long-range regulatory elements spread over 120 kilobases on chromosome 5q31. [provided by RefSeq, Jul 2013] |
| UniProt Code: | P05113 |
| NCBI GenInfo Identifier: | 124341 |
| NCBI Gene ID: | 3567 |
| NCBI Accession: | P05113.1 |
| UniProt Secondary Accession: | P05113,Q13840, |
| UniProt Related Accession: | P05113 |
| Molecular Weight: | 15,238 Da |
| NCBI Full Name: | Interleukin-5 |
| NCBI Synonym Full Names: | interleukin 5 |
| NCBI Official Symbol: | IL5�� |
| NCBI Official Synonym Symbols: | EDF; TRF; IL-5�� |
| NCBI Protein Information: | interleukin-5 |
| UniProt Protein Name: | Interleukin-5 |
| UniProt Synonym Protein Names: | B-cell differentiation factor I; Eosinophil differentiation factor; T-cell replacing factor; TRF |
| Protein Family: | Interleukin |
| UniProt Gene Name: | IL5�� |
| UniProt Entry Name: | IL5_HUMAN |
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