The Human Integrin Alpha 1 (ITGA1) ELISA Kit is specifically designed for the reliable and accurate detection of ITGA1 levels in human samples, including serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring consistent and reproducible results for a variety of research applications.Integrin Alpha 1 is a key protein involved in cell adhesion and migration, playing a crucial role in processes such as tissue repair, inflammation, and tumor progression. By measuring ITGA1 levels, researchers can gain insight into various diseases and conditions, including cancer, fibrosis, and inflammatory disorders.
This ELISA kit provides a valuable tool for studying ITGA1 expression and its potential implications in disease pathology and therapeutic interventions.Overall, the Human Integrin Alpha 1 (ITGA1) ELISA Kit offers a reliable and efficient method for quantifying ITGA1 levels in human samples, making it a valuable asset for researchers interested in understanding the role of this protein in health and disease.
Product Name:
Human Integrin Alpha 1 (ITGa1) ELISA Kit
Product Code:
HUDL01563
Size:
96 Assays
Target:
ITGa1
Synonyms:
CD49-A, CD49a, ITG-A1, VLA1, CD49 antigen-like family member A, Laminin and collagen receptor
Detection Method:
Sandwich
Range:
78.125-5,000pg/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
