Human Killer Cell Immunoglobulin Like Receptor 2DS4 (KIR2DS4) ELISA Kit
The Human Killer Cell Immunoglobulin-Like Receptor 2DS4 (KIR2DS4) ELISA Kit is a powerful tool for accurately measuring levels of KIR2DS4 in human samples. This kit is specifically designed for the detection of KIR2DS4 in serum, plasma, and cell culture supernatants with high sensitivity and specificity.Killer cell immunoglobulin-like receptors (KIRs) are important regulators of immune response and play a critical role in the recognition of target cells. KIR2DS4 is a specific KIR protein that is associated with various immune-related diseases and conditions.
By using this ELISA kit, researchers can reliably and reproducibly measure KIR2DS4 levels, which can provide valuable insights into immune responses and potential therapeutic targets. This kit is essential for research in immunology, oncology, and infectious diseases, offering a comprehensive solution for studying KIR2DS4 and its implications in human health.
Product Name:
Human Killer Cell Immunoglobulin Like Receptor 2DS4 (KIR2DS4) ELISA Kit
Product Code:
HUDL01613
Size:
96 Assays
Target:
KIR2DS4
Synonyms:
CD158I, KIR1D, KIR412, KKA3, NKAT8, PAX, Cl-39, Natural killer-associated transcript 8, CD158 antigen-like family member I, NK receptor CL-39/CL-17
Detection Method:
Sandwich
Range:
0.156-10ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-101
91
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
