Human MAPK13 Recombinant Protein (RPPB2574)
- SKU:
- RPPB2574
- Product Type:
- Recombinant Protein
- Species:
- Human
- Uniprot:
- O15264
- Research Area:
- Enzymes
Description
| Product Name: | Human MAPK13 Recombinant Protein |
| Product Code: | RPPB2574 |
| Size: | 20µg |
| Species: | Human |
| Target: | MAPK13 |
| Synonyms: | Mitogen-activated protein kinase 13, PRKM13, SAPK4, p38delta, Mitogen-activated protein kinase p38 delta, Stress-activated protein kinase 4, MAP kinase 13, MAP kinase p38 delta, EC 2.7.11.24. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile Filtered colorless solution. |
| Formulation: | The MAPK13 solution (1mg/ml) contains 20mM Tris-HCl buffer (pH 8.0), 0.1M NaCl, 1mM DTT and 20% glycerol. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Purity: | Greater than 90% as determined by SDS-PAGE. |
| Amino Acid Sequence: | MGSSHHHHHH SSGLVPRGSH MSLIRKKGFY KQDVNKTAWE LPKTYVSPTH VGSGAYGSVC SAIDKRSGEK VAIKKLSRPF QSEIFAKRAY RELLLLKHMQ HENVIGLLDV FTPASSLRNF YDFYLVMPFM QTDLQKIMGM EFSEEKIQYL VYQMLKGLKY IHSAGVVHRD LKPGNLAVNE DCELKILDFG LARHADAEMT GYVVTRWYRA PEVILSWMHY NQTVDIWSVG CIMAEMLTGK TLFKGKDYLD QLTQILKVTG VPGTEFVQKL NDKAAKSYIQ SLPQTPRKDF TQLFPRASPQ AADLLEKMLE LDVDKRLTAA QALTHPFFEP FRDPEEETEA QQPFDDSLEH EKLTVDEWKQ HIYKEIVNFS PIARKDSRRR SGMKL |
MAPK13 belongs to the MAP kinase family. MAP kinases perform as an incorporation point for various biochemical signals. MAP kinases take part in an extensive range of cellular courses like differentiation, development, transcription regulation and proliferation. MAPK13 is phosphorylated by MKK6 as a reaction to cytokines and cellular stresses.
MAPK13 Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 385 amino acids (1-365) and having a molecular mass of 44.2 kDa.MAPK13 is fused to a 20 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
| UniProt Protein Function: | Function: Serine/threonine kinase which acts as an essential component of the MAP kinase signal transduction pathway. MAPK13 is one of the four p38 MAPKs which play an important role in the cascades of cellular responses evoked by extracellular stimuli such as proinflammatory cytokines or physical stress leading to direct activation of transcription factors such as ELK1 and ATF2. Accordingly, p38 MAPKs phosphorylate a broad range of proteins and it has been estimated that they may have approximately 200 to 300 substrates each. MAPK13 is one of the less studied p38 MAPK isoforms. Some of the targets are downstream kinases such as MAPKAPK2, which are activated through phosphorylation and further phosphorylate additional targets. Plays a role in the regulation of protein translation by phosphorylating and inactivating EEF2K. Involved in cytoskeletal remodeling through phosphorylation of MAPT and STMN1. Mediates UV irradiation induced up-regulation of the gene expression of CXCL14. Plays an important role in the regulation of epidermal keratinocyte differentiation, apoptosis and skin tumor development. Phosphorylates the transcriptional activator MYB in response to stress which leads to rapid MYB degradation via a proteasome-dependent pathway. MAPK13 also phosphorylates and down-regulates PRKD1 during regulation of insulin secretion in pancreatic beta cells. Ref.12 Ref.15 Ref.16 Ref.17 Ref.18 Ref.19 Ref.21 Ref.23 |
| UniProt Protein Details: | Catalytic activity: ATP + a protein = ADP + a phosphoprotein. Cofactor: Magnesium. Enzyme regulation: Activated by phosphorylation on threonine and tyrosine by dual specificity kinases, MAP2K3/MKK3, MAP2K6/MKK6, MAP2K4/MKK4 and MAP2K7/MKK7. Activation by ultraviolet radiation, hyperosmotic shock, anisomycin or by TNF-alpha is mediated by MAP2K3/MKK3. Inhibited by dual specificity phosphatase DUSP1. Ref.1 Ref.2 Ref.15 Subunit structure: Interacts with MAPK8IP2. Ref.14 Tissue specificity: Expressed in testes, pancreas, small intestine, lung and kidney. Abundant in macrophages, also present in neutrophils, CD4+ T-cells, and endothelial cells. Ref.2 Ref.13 Domain: The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases. Post-translational modification: Dually phosphorylated on Thr-180 and Tyr-182 by MAP2K3/MKK3, MAP2K4/MKK4, MAP2K6/MKK6 and MAP2K7/MKK7, which activates the enzyme. Dephosphorylated by dual specificity phosphatase DUSP1. Ref.2 Ref.21 Sequence similarities: Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily.Contains 1 protein kinase domain. |
| NCBI Summary: | This gene encodes a member of the mitogen-activated protein (MAP) kinase family. MAP kinases act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. The encoded protein is a p38 MAP kinase and is activated by proinflammatory cytokines and cellular stress. Substrates of the encoded protein include the transcription factor ATF2 and the microtubule dynamics regulator stathmin. Alternatively spliced transcript variants have been observed for this gene. [provided by RefSeq, Jul 2012] |
| UniProt Code: | O15264 |
| NCBI GenInfo Identifier: | 6685642 |
| NCBI Gene ID: | 5603 |
| NCBI Accession: | O15264.1 |
| UniProt Secondary Accession: | O15264,O14739, O15124, Q6FI46, Q9UNU0, |
| UniProt Related Accession: | O15264 |
| Molecular Weight: | 42,090 Da |
| NCBI Full Name: | Mitogen-activated protein kinase 13 |
| NCBI Synonym Full Names: | mitogen-activated protein kinase 13 |
| NCBI Official Symbol: | MAPK13�� |
| NCBI Official Synonym Symbols: | SAPK4; PRKM13; MAPK 13; MAPK-13; p38delta�� |
| NCBI Protein Information: | mitogen-activated protein kinase 13; MAP kinase 13; MAP kinase p38 delta; stress-activated protein kinase 4; mitogen-activated protein kinase p38 delta |
| UniProt Protein Name: | Mitogen-activated protein kinase 13 |
| UniProt Synonym Protein Names: | Mitogen-activated protein kinase p38 delta; MAP kinase p38 delta; Stress-activated protein kinase 4 |
| UniProt Gene Name: | MAPK13�� |
| UniProt Entry Name: | MK13_HUMAN |
