SynonymsCCL2,GDCF2,HC11,HSMCR30,MCAF,MCP1,SCYA2,SMC-CF,Chemokine C-C-Motif Ligand 2,Monocyte Chemotactic And Activating Factor,Monocyte Secretory Protein JE
ReactivityHuman
Sample TypeSerum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernatants And Other Biological Fluids
SKU:
HUDL01814
UPC:
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
P13500
Sensitivity:
6.6pg/mL
Range:
15.625-1000pg/mL
ELISA Type:
Sandwich
Synonyms:
CCL2,GDCF2,HC11,HSMCR30,MCAF,MCP1,SCYA2,SMC-CF,Chemokine C-C-Motif Ligand 2,Monocyte Chemotactic And Activating Factor,Monocyte Secretory Protein JE
Reactivity:
Human
Sample Type:
Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernatants And Other Biological Fluids
Human Monocyte Chemotactic Protein 1 (MCP1) ELISA Kit
The Human Monocyte Chemotactic Protein-1 (MCP-1) ELISA Kit is a powerful tool for accurate detection of MCP-1 levels in human samples including serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit ensures precise and reproducible results, making it an excellent choice for a wide array of research applications.MCP-1 is a key chemokine that plays a crucial role in the recruitment of monocytes to sites of inflammation, contributing to various inflammatory diseases and conditions such as atherosclerosis, arthritis, and certain types of cancer.
By measuring MCP-1 levels, researchers can gain valuable insights into the inflammatory processes underlying these diseases, aiding in the development of targeted therapies and interventions.
Product Name:
Human Monocyte Chemotactic Protein 1 (MCP1) ELISA Kit
Product Code:
HUDL01814
Size:
96 Assays
Target:
MCP1
Synonyms:
CCL2, GDCF2, HC11, HSMCR30, MCAF, MCP1, SCYA2, SMC-CF, Chemokine C-C-Motif Ligand 2, Monocyte Chemotactic And Activating Factor, Monocyte Secretory Protein JE
Detection Method:
Sandwich
Range:
15.625-1,000pg/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
