Human NAT1 Recombinant Protein (RPPB2001)
- SKU:
- RPPB2001
- Product Type:
- Recombinant Protein
- Species:
- Human
- Uniprot:
- P18440
- Research Area:
- Enzymes
Description
| Product Name: | Human NAT1 Recombinant Protein |
| Product Code: | RPPB2001 |
| Size: | 20µg |
| Species: | Human |
| Target: | NAT1 |
| Synonyms: | N-acetyltransferase 1 (arylamine N-acetyltransferase), AAC1, Arylamide acetylase 1, Monomorphic arylamine N-acetyltransferase, MNAT, NAT-1. |
| Source: | Escherichia Coli |
| Physical Appearance: | NAT1 is supplied as a sterile filtered clear solution. |
| Formulation: | NAT1 protein 0.5mg/ml is supplied in 20mM Tris-HCl, pH-8, 0.1M NaCl, 2mM DTT and 10% Glycerol. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Purity: | Greater than 85.0% as determined by SDS-PAGE. |
| Amino Acid Sequence: | MGSSHHHHHH SSGLVPRGSH MDIEAYLERI GYKKSRNKLD LETLTDILQH QIRAVPFENL NIHCGDAMDL GLEAIFDQVV RRNRGGWCLQ VNHLLYWALT TIGFETTMLG GYVYSTPAKK YSTGMIHLLL QVTIDGRNYI VDAGFGRSYQ MWQPLELISG KDQPQVPCIF RLTEENGFWY LDQIRREQYI PNEEFLHSDL LEDSKYRKIY SFTLKPRTIE DFESMNTYLQ TSPASVFTSK SFCSLQTPDG VHCLVGFTLT HRRFNYKDNT DLIEFKTLSE EEIEKVLKNI FNISLQRKLV PKHGDRFFTI |
NAT1 is a member of the arylamine N-acetyltransferase family. NAT1 catalyzes N- or O-acetylation of heterocyclic and arylamine substrates in the detoxification of a large number of drugs. Several alleles triggering high levels of N-acetyltransferase activity were linked to colon and urinary bladder cancers, since NAT1 also bioactivate numerous known carcinogens. NAT1 aids metabolize drugs and other xenobiotics, and takes part in the detoxification of a plethora of hydrazine and arylamine drugs.
NAT1 Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 310 amino acids (1-290) and having a molecular mass of 36.1 kDa.The NAT1 is fused to a 20 amino acid His-Tag at N-terminus and purified by proprietary chromatographic techniques.
| UniProt Protein Function: | NAT1: Participates in the detoxification of a plethora of hydrazine and arylamine drugs. Catalyzes the N- or O-acetylation of various arylamine and heterocyclic amine substrates and is able to bioactivate several known carcinogens. Belongs to the arylamine N-acetyltransferase family. |
| UniProt Protein Details: | Protein type:EC 2.3.1.5; Acetyltransferase; Xenobiotic Metabolism - drug metabolism - other enzymes; Secondary Metabolites Metabolism - caffeine Chromosomal Location of Human Ortholog: 8p22 Cellular Component: cytosol Molecular Function:arylamine N-acetyltransferase activity Biological Process: xenobiotic metabolic process |
| NCBI Summary: | This gene is one of two arylamine N-acetyltransferase (NAT) genes in the human genome, and is orthologous to the mouse and rat Nat2 genes. The enzyme encoded by this gene catalyzes the transfer of an acetyl group from acetyl-CoA to various arylamine and hydrazine substrates. This enzyme helps metabolize drugs and other xenobiotics, and functions in folate catabolism. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2011] |
| UniProt Code: | P18440 |
| NCBI GenInfo Identifier: | 114234 |
| NCBI Gene ID: | 9 |
| NCBI Accession: | P18440.2 |
| UniProt Secondary Accession: | P18440,O15159, O15300, Q546N1, Q96TE9, A8K4E7, |
| UniProt Related Accession: | P18440 |
| Molecular Weight: | 33,899 Da |
| NCBI Full Name: | Arylamine N-acetyltransferase 1 |
| NCBI Synonym Full Names: | N-acetyltransferase 1 (arylamine N-acetyltransferase) |
| NCBI Official Symbol: | NAT1�� |
| NCBI Official Synonym Symbols: | AAC1; MNAT; NATI; NAT-1�� |
| NCBI Protein Information: | arylamine N-acetyltransferase 1; arylamide acetylase 1; N-acetyltransferase type 1; monomorphic arylamine N-acetyltransferase |
| UniProt Protein Name: | Arylamine N-acetyltransferase 1 |
| UniProt Synonym Protein Names: | Arylamide acetylase 1; Monomorphic arylamine N-acetyltransferase; MNAT; N-acetyltransferase type 1; NAT-1 |
| Protein Family: | Arylamine N-acetyltransferase |
| UniProt Gene Name: | NAT1�� |
| UniProt Entry Name: | ARY1_HUMAN |
