Human NOV Recombinant Protein (RPPB0806)
- SKU:
- RPPB0806
- Product Type:
- Recombinant Protein
- Species:
- Human
- Uniprot:
- P48745
- Research Area:
- Growth Factors & Cytokines
Description
| Product Name: | Human NOV Recombinant Protein |
| Product Code: | RPPB0806 |
| Size: | 20µg |
| Species: | Human |
| Target: | NOV |
| Synonyms: | Protein NOV homolog, NovH, CCN family member 3, nsulin-like growth factor-binding protein 9, IBP-9, IGF-binding protein 9, IGFBP-9, Nephroblastoma-overexpressed gene protein homolog, NOV, CCN3, IGFBP9, NOVH. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile Filtered White lyophilized (freeze-dried) powder. |
| Formulation: | Lyophilized from a 0.2�m filtered concentrated solution in 20mM Tris-HCl, pH 8.6 and 150 mM NaCl. |
| Solubility: | It is recommended to reconstitute the lyophilized NOV in sterile 18M-cm H2O not less than 100�g/ml, which can then be further diluted to other aqueous solutions. |
| Stability: | Lyophilized NOV although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution NOV should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles. |
| Purity: | Greater than 95.0% as determined by:(a) Analysis by RP-HPLC.(b) Analysis by SDS-PAGE. |
| Amino Acid Sequence: | MQVAATQRCP PQCPGRCPAT PPTCAPGVRA VLDGCSCCLV CARQRGESCS DLEPCDESSG LYCDRSADPS NQTGICTAVE GDNCVFDGVI YRSGEKFQPS CKFQCTCRDG QIGCVPRCQL DVLLPEPNCP APRKVEVPGE CCEKWICGPD EEDSLGGLTL AAYRPEATLG VEVSDSSVNC IEQTTEWTAC SKSCGMGFST RVTNRNRQCE MLKQTRLCMV RPCEQEPEQP TDKKGKKCLR TKKSLKAIHL QFKNCTSLHT YKPRFCGVCS DGRCCTPHNT KTIQAEFQCS PGQIVKKPVM VIGTCTCHTN CPKNNEAFLQ ELELKTTRGK M |
| Biological Activity: | Determined by a cell proliferation assay using murine Balb/c 3T3 cells is less than 1.0 �g/ml, corresponding to a specific activity of > 1000 IU/mg. range of 10.0 -50.0 ng/ml, corresponding to a specific activity of 20,000-100,000units/mg. |
Nephroblastoma Overexpressed (NOV) which is encoded by the NOV gene is a part of the CCN (CTGF/CYR61/NOV) family. NOV takes part in reducing tumorgenicity and proliferation of certain cancer cell lines. NOV interacts with numerous proteins and is involved in both internal and external cell signaling. NOV is expressed in particular tumors, including Wilm�s tumor and most nephroblastomas and is also exerts proangiogenic activities.
Nephroblastoma Overexpressed Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 331 amino acids and having a molecular mass of 36.2 kDa. The NOV is purified by proprietary chromatographic techniques.
| UniProt Protein Function: | NOV: Immediate-early protein likely to play a role in cell growth regulation. Belongs to the CCN family. Expression is down-regulated by WT1. Interacts with FBLN1. |
| UniProt Protein Details: | Protein type:Secreted; Secreted, signal peptide Chromosomal Location of Human Ortholog: 8q24.1 Cellular Component: proteinaceous extracellular matrix; cell soma; intracellular membrane-bound organelle; axon; dendrite Molecular Function:heparin binding; integrin binding; insulin-like growth factor binding; growth factor activity Biological Process: cell-cell signaling; regulation of gene expression; regulation of cell growth; cell adhesion; signal transduction |
| NCBI Summary: | The protein encoded by this gene is a small secreted cysteine-rich protein and a member of the CCN family of regulatory proteins. CNN family proteins associate with the extracellular matrix and play an important role in cardiovascular and skeletal development, fibrosis and cancer development. [provided by RefSeq, Feb 2009] |
| UniProt Code: | P48745 |
| NCBI GenInfo Identifier: | 1352515 |
| NCBI Gene ID: | 4856 |
| NCBI Accession: | P48745.1 |
| UniProt Secondary Accession: | P48745,Q6I9S3, Q96BY5, Q9UDE4, B2R5X7, |
| UniProt Related Accession: | P48745 |
| Molecular Weight: | 39,162 Da |
| NCBI Full Name: | Protein NOV homolog |
| NCBI Synonym Full Names: | nephroblastoma overexpressed |
| NCBI Official Symbol: | NOV�� |
| NCBI Official Synonym Symbols: | CCN3; NOVh; IBP-9; IGFBP9; IGFBP-9�� |
| NCBI Protein Information: | protein NOV homolog; CCN family member 3; IGF-binding protein 9; insulin-like growth factor-binding protein 9; nephroblastoma-overexpressed gene protein homolog |
| UniProt Protein Name: | Protein NOV homolog |
| UniProt Synonym Protein Names: | CCN family member 3; Insulin-like growth factor-binding protein 9; IBP-9; IGF-binding protein 9; IGFBP-9; Nephroblastoma-overexpressed gene protein homolog |
| Protein Family: | Protein |
| UniProt Gene Name: | NOV�� |
| UniProt Entry Name: | NOV_HUMAN |
