The Human NTN4 (Netrin-4) CLIA Kit is specifically designed for the quantitative detection of Netrin-4 levels in human serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, providing accurate and reproducible results for various research applications.Netrin-4 is a key protein involved in axon guidance and neuronal development, playing a critical role in the formation of neural circuits. Dysregulation of Netrin-4 has been associated with neurological disorders such as autism, schizophrenia, and Alzheimer's disease, making it a valuable biomarker for studying these conditions and exploring potential therapeutic interventions.
Overall, the Human NTN4 (Netrin-4) CLIA Kit is a reliable tool for researchers looking to investigate the role of Netrin-4 in neurological development and pathology, with the potential to contribute to advances in diagnostics and treatment strategies for related disorders.
Product Name:
Human Ntn4 (Netrin 4) CLIA Kit
SKU:
HUES01206
Target:
Human Ntn4 (Netrin 4)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human Ntn4. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human Ntn4 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human Ntn4, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human Ntn4. You can calculate the concentration of Human Ntn4 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
101-118
84-97
86-100
Average (%)
107
91
93
1:4
Range (%)
99-115
102-116
87-101
Average (%)
105
110
94
1:8
Range (%)
90-102
99-114
98-112
Average (%)
97
107
104
1:16
Range (%)
99-117
94-107
97-111
Average (%)
108
99
104
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
87-101
92
EDTA plasma (n=5)
91-106
97
Cell culture media (n=5)
91-101
96
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
50.95
150.7
453.52
55.38
151.71
459.89
Standard deviation
6.4
15.16
50.43
4.77
15.75
50.54
C V (%)
12.56
10.06
11.12
8.61
10.38
10.99
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human Ntn4 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human Ntn4 in samples. No significant cross-reactivity or interference between Human Ntn4 and analogues was observed.
