Human Nuclear apoptosis-inducing factor 1/NAIF1 ELISA Kit
The Human Nuclear Apoptosis-Inducing Factor 1 (NAIF1) ELISA Kit is a cutting-edge tool designed for the precise measurement of NAIF1 levels in human samples including serum, plasma, and cell culture supernatants. With its exceptional sensitivity and specificity, this kit delivers reliable and consistent results, making it an invaluable asset for various research purposes. NAIF1 is a vital protein involved in the regulation of apoptosis, the process of programmed cell death. It plays a crucial role in influencing cell survival and death pathways, making it a key player in various biological processes.
Understanding the function of NAIF1 can provide valuable insights into conditions involving abnormal cell death, such as cancer, neurodegenerative disorders, and immune-related diseases. By utilizing the Human NAIF1 ELISA Kit, researchers can uncover the role of NAIF1 in different cellular processes and disease states, paving the way for the development of novel therapeutic strategies and potential diagnostic tools. This kit is an essential tool for anyone studying apoptosis and its implications in human health and disease.
Product Name:
Human Nuclear apoptosis-inducing factor 1/NAIF1 ELISA Kit
SKU:
HUFI00962
Reactivity:
Human
Assay Type:
Sandwich ELISA, Double Antibody
Sensitivity:
0.188 ng/mL
Range:
0.313-20 ng/mL
Sample Type:
Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate, Other Liquid Samples
Storage:
2-8°C for 12 months.
Linearity:
Sample
1:2
1:4
1:8
Serum (n = 5)
86-101%
84-100%
80-97%
EDTA Plasma (n = 5)
89-100%
87-101%
81-99%
Heparin Plasma (n = 5)
86-102%
89-98%
81-96%
Recovery:
Sample
Recovery Range (%)
Average (%)
Serum (n = 5)
93-102
99
EDTA Plasma (n = 5)
85-105
95
Heparin Plasma (n = 5)
93-105
99
Note:The below protocol is a sample protocol. Protocols are specific to each batch/lot. For the correct instructions please follow the protocol included in your kit.
Step
Procedure
1
Reagent & Plate Preparation: Equilibrate reagents and TMB substrate to room temperature. Set standard, test sample and control (zero) wells on the pre-coated plate and record their positions.
2
Primary Incubation: Prepare standards, samples, blanks and load into designated wells. Incubate plate at 37°C for 90 minutes to allow antigen binding.
3
Detection Antibody Binding: Add biotin-labeled detection antibody and incubate at 37°C for 60 minutes.
4
HRP-Streptavidin Binding: Add HRP-Streptavidin (SABC) and incubate at 37°C for 30 minutes.
5
Color Development: Add TMB substrate and incubate in the dark for 10–20 minutes.
6
Stop Reaction & Reading: Add stop solution and measure absorbance at 450 nm immediately.
Sample Type
Protocol
Serum
Allow blood to clot, centrifuge at 1000 × g for 20 minutes, collect supernatant supernatant and store appropriately.
Plasma
Collect using anticoagulant tubes, centrifuge at 1000 × g for 15 minutes at 2–8°C and collect plasma.
Tissue Homogenate
Homogenize tissue in PBS with protease inhibitors, centrifuge and collect supernatant.
Cell Culture Supernatant
Centrifuge at 2500 rpm for 5 minutes and collect clarified supernatant.
Cell Lysate
Lyse cells using lysis buffer with protease inhibitors, centrifuge and collect protein supernatant.
Other Sample Types
For more information about how to process other sample types, (e.g., body fluids, breast milk & more), please contact our Tech Support Team at techsupport@assaygenie.com.
Component
Quantity
Storage
48T
96T
ELISA Microplate (Dismountable)
8×6
8×12
Place the test strips into a sealed foil bag with the desiccant. Store for 1 month at 2-8°C; Store for 12 months at -20°C.
Lyophilized Standard
1 vial
2 vial
Place the standards into a sealed foil bag with the desiccant. Store for 1 month at 2-8°C; Store for 12 months at -20°C.
