Human PAPP A Recombinant Protein (RPPB2069)
- SKU:
- RPPB2069
- Product Type:
- Recombinant Protein
- Species:
- Human
- Uniprot:
- Q13219
- Research Area:
- Enzymes
Description
| Product Name: | Human PAPP A Recombinant Protein |
| Product Code: | RPPB2069 |
| Size: | 10µg |
| Species: | Human |
| Target: | PAPP A |
| Synonyms: | Pappalysin-1, Pregnancy-associated plasma protein A, PAPP-A, IGF-dependent IGFBP-4 protease, IGFBP-4ase, PAPPA, PAPA, DIPLA1, PAPPA1, ASBABP2. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile Filtered clear solution. |
| Formulation: | PAPP-A protein is supplied in 1x PBS and 50% glycerol. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. Please avoid freeze thaw cycles. |
| Purity: | Greater than 95.0% as determined by SDS-PAGE. |
PAPPA is a large zinc binding protein, which acts as a metalloprotease and specifically cleaves IGFBP-4 and IGFBP-5, resulting in release of bound IGF. PAPP-A can also act as a regulator of IGF bioactivity in a number of biological systems, including the human ovary and cardiovascular systems. It was shown that PAPP A levels are elevated in patients with unstable angina or acute myocardial infarction. Furthermore, PAPPA is believed to be involved in local proliferative processes such as wound healing and bone remodeling. Moreover, PAPP-A is produced in high concentrations during pregnancy and is released into the maternal circulation. In placenta, PAPP A is expressed in X cells in septa and anchoring villi, and in syncytiotrophoblasts in the chorionic villi. Lower levels of PAPPA are found in an array of other tissues including kidney, myometrium, endometrium, ovaries, breast, prostate, bone marrow, colon, fibroblasts and osteoblasts. PAPP-A is present in serum and placenta during pregnancy; with levels increasing throughout pregnancy. Low levels of PAPP A are associated with a number of foetal chromosomal abnormalities, as well as pre-eclampsia and stillbirth.PAPPA levels may be a potentially highly specific marker for heart disease.PAPP-A proteolytic activity is inhibited by targeting substrate exosite binding.
PAPP-A Human Recombinant produced in E.Coli is single, a non-glycosylated, Polypeptide chain containing 191 amino acids fragment (81-271) corresponding to the PAPP-A �Jelly-Roll� domain fragment, having a total molecular mass of 30kDa and fused with a 4.5kDa amino-terminal hexahistidine tag. The PAPP-A is purified by proprietary chromatographic techniques.
| UniProt Protein Function: | PAPPA: Metalloproteinase which specifically cleaves IGFBP-4 and IGFBP-5, resulting in release of bound IGF. Cleavage of IGFBP-4 is dramatically enhanced by the presence of IGF, whereas cleavage of IGFBP-5 is slightly inhibited by the presence of IGF. Belongs to the peptidase M43B family. |
| UniProt Protein Details: | Protein type:EC 3.4.24.79; Secreted; Secreted, signal peptide; Protease Chromosomal Location of Human Ortholog: 9q33.2 Cellular Component: membrane; extracellular region Molecular Function:metallopeptidase activity; zinc ion binding; metalloendopeptidase activity Biological Process: cellular protein metabolic process; female pregnancy; cell differentiation; proteolysis |
| NCBI Summary: | This gene encodes a secreted metalloproteinase which cleaves insulin-like growth factor binding proteins (IGFBPs). Following IGFBP cleavage, insulin growth factors dissociate from IGFBPs and bind to IGF receptors, resulting in activation of the IGF pathway. The encoded protein plays a role in bone formation, inflammation, wound healing and female fertility. Enhanced expression of this protein is associated with diabetic nephropathy in human patients and this protein may promote tumor invasion and growth in various human cancers. [provided by RefSeq, Aug 2017] |
| UniProt Code: | Q13219 |
| NCBI GenInfo Identifier: | 223590248 |
| NCBI Gene ID: | 5069 |
| NCBI Accession: | Q13219.3 |
| UniProt Related Accession: | Q13219 |
| Molecular Weight: | Predicted MW: 25.9kDaAccurate MW: 33kDa |
| NCBI Full Name: | Pappalysin-1 |
| NCBI Synonym Full Names: | pappalysin 1 |
| NCBI Official Symbol: | PAPPA�� |
| NCBI Official Synonym Symbols: | PAPA; DIPLA1; PAPP-A; PAPPA1; ASBABP2; IGFBP-4ase�� |
| NCBI Protein Information: | pappalysin-1 |
| UniProt Protein Name: | Pappalysin-1 |
| UniProt Synonym Protein Names: | Insulin-like growth factor-dependent IGF-binding protein 4 protease; IGF-dependent IGFBP-4 protease; IGFBP-4ase; Pregnancy-associated plasma protein A |
| Protein Family: | Pappalysin |
| UniProt Gene Name: | PAPPA�� |
| UniProt Entry Name: | PAPP1_HUMAN |
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