The Human PDGFR-beta (Platelet-Derived Growth Factor Receptor Beta) CLIA Kit is specifically designed for the accurate quantification of PDGFR-beta levels in human serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring precise and consistent results for various research applications.PDGFR-beta is a vital receptor involved in cell growth, proliferation, and differentiation, playing a crucial role in various physiological and pathological processes.
Abnormalities in PDGFR-beta signaling have been linked to conditions such as cancer, fibrosis, and cardiovascular diseases, making it a valuable biomarker for studying these diseases and developing potential therapeutic interventions.Overall, the Human PDGFR-beta CLIA Kit provides researchers with a reliable tool for studying PDGFR-beta expression levels and signaling pathways, ultimately enhancing our understanding of the role of PDGFR-beta in health and disease.
Human PDGFR beta (Platelet Derived Growth Factor Receptor Beta)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human PDGFRβ. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human PDGFRβ and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human PDGFRβ, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human PDGFRβ. You can calculate the concentration of Human PDGFRβ in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
85-96
89-100
91-102
Average (%)
91
95
97
1:4
Range (%)
99-113
91-102
89-100
Average (%)
107
96
95
1:8
Range (%)
95-111
101-118
98-113
Average (%)
102
108
105
1:16
Range (%)
98-113
85-100
94-110
Average (%)
105
91
102
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
90-103
97
EDTA plasma (n=5)
89-101
94
Cell culture media (n=5)
86-99
91
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
106.49
212.77
704.78
97.43
215.77
727.0
Standard deviation
10.92
15.3
61.25
11.23
21.38
68.19
C V (%)
10.25
7.19
8.69
11.53
9.91
9.38
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human PDGFRβ concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human PDGFRβ in samples. No significant cross-reactivity or interference between Human PDGFRβ and analogues was observed.
