Human PDI Recombinant Protein (RPPB2081)
- SKU:
- RPPB2081
- Product Type:
- Recombinant Protein
- Species:
- Human
- Uniprot:
- P07237
- Research Area:
- Enzymes
Description
| Product Name: | Human PDI Recombinant Protein |
| Product Code: | RPPB2081 |
| Size: | 0.5mg |
| Species: | Human |
| Target: | PDI |
| Synonyms: | Protein Disulfide Isomerase, PDI, EC 5.3.4.1, Prolyl 4-hydroxylase subunit beta, Cellular thyroid hormone-binding protein, p55, P4HB, ERBA2L, PDIA1, PO4DB, DSI, GIT, PHDB, PO4HB, PROHB, P4Hbeta. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile Filtered White lyophilized (freeze-dried) powder. |
| Formulation: | The PDI protein (1mg/ml)solution was lyophilized from PBS pH-7. |
| Solubility: | It is recommended to reconstitute the lyophilized PDI in sterile 18M?-cm H2O not less than 100�g/ml, which can then be further diluted to other aqueous solutions. |
| Stability: | Lyophilized Protein Disulfide Isomerase although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution Human PDI should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please avoid freeze-thaw cycles. |
| Purity: | Greater than 95.0% as determined by:a) Analysis by RP-HPLC.b) Analysis by SDS-PAGE. |
| Amino Acid Sequence: | The sequence of the first five N-terminal amino acids was determined and was found to be Met-Leu-Arg-Arg-Ala |
Protein disulfide isomerases (PDIs) constitute a family of structurally related enzymes which catalyze disulfide bonds formation, reduction, or isomerization of newly synthesized proteins in the lumen of the endoplasmic reticulum (ER). They act also as chaperones, and are, therefore, part of a quality-control system for the correct folding of the proteins in the same subcellular compartment. PDI has been found to have moderate effects (25-fold) on the rate of oxidative folding of proteins in vitro.Recombinant Human Protein Disulfide Isomerase is involved in disulphide-bond formation and isomerization, as well as the reduction of disulphide bonds in proteins. Recombinant PDI has been found to have moderate effects (25-fold) on the rate of oxidative folding of proteins in vitro.
PDI Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 503 amino acids and having a molecular mass of 62.4 kDa. The PDI is fused to a 12 amino acid His tag (515 a.a. total) at N-terminal and purified by proprietary chromatographic techniques.
| UniProt Protein Function: | PDIA1: This multifunctional protein catalyzes the formation, breakage and rearrangement of disulfide bonds. At the cell surface, seems to act as a reductase that cleaves disulfide bonds of proteins attached to the cell. May therefore cause structural modifications of exofacial proteins. Inside the cell, seems to form/rearrange disulfide bonds of nascent proteins. At high concentrations, functions as a chaperone that inhibits aggregation of misfolded proteins. At low concentrations, facilitates aggregation (anti-chaperone activity). May be involved with other chaperones in the structural modification of the TG precursor in hormone biogenesis. Also acts a structural subunit of various enzymes such as prolyl 4-hydroxylase and microsomal triacylglycerol transfer protein MTTP. Homodimer. Monomers and homotetramers may also occur. Also constitutes the structural subunit of prolyl 4-hydroxylase and of the microsomal triacylglycerol transfer protein MTTP in mammalian cells. Stabilizes both enzymes and retain them in the ER without contributing to the catalytic activity. Binds UBQLN1. Binds to CD4, and upon HIV-1 binding to the cell membrane, is part of a P4HB/PDI-CD4-CXCR4-gp120 complex. Belongs to the protein disulfide isomerase family. |
| UniProt Protein Details: | Protein type:EC 5.3.4.1; Nuclear receptor co-regulator; Isomerase; Oxidoreductase; Endoplasmic reticulum Chromosomal Location of Human Ortholog: 17q25 Cellular Component: focal adhesion; endoplasmic reticulum lumen; endoplasmic reticulum; ER-Golgi intermediate compartment; plasma membrane; extracellular region; melanosome Molecular Function:protein binding; enzyme binding; procollagen-proline 4-dioxygenase activity; protein heterodimerization activity; endopeptidase activity; protein disulfide isomerase activity Biological Process: response to reactive oxygen species; extracellular matrix organization and biogenesis; protein folding; cell redox homeostasis; lipoprotein metabolic process; peptidyl-proline hydroxylation to 4-hydroxy-L-proline; proteolysis Disease: Cole-carpenter Syndrome 1 |
| NCBI Summary: | This gene encodes the beta subunit of prolyl 4-hydroxylase, a highly abundant multifunctional enzyme that belongs to the protein disulfide isomerase family. When present as a tetramer consisting of two alpha and two beta subunits, this enzyme is involved in hydroxylation of prolyl residues in preprocollagen. This enzyme is also a disulfide isomerase containing two thioredoxin domains that catalyze the formation, breakage and rearrangement of disulfide bonds. Other known functions include its ability to act as a chaperone that inhibits aggregation of misfolded proteins in a concentration-dependent manner, its ability to bind thyroid hormone, its role in both the influx and efflux of S-nitrosothiol-bound nitric oxide, and its function as a subunit of the microsomal triglyceride transfer protein complex. [provided by RefSeq, Jul 2008] |
| UniProt Code: | P07237 |
| NCBI GenInfo Identifier: | 2507460 |
| NCBI Gene ID: | 5034 |
| NCBI Accession: | P07237.3 |
| UniProt Secondary Accession: | P07237,P30037, P32079, Q15205, Q6LDE5, B2RDQ2, |
| UniProt Related Accession: | P07237 |
| Molecular Weight: | 57,116 Da |
| NCBI Full Name: | Protein disulfide-isomerase |
| NCBI Synonym Full Names: | prolyl 4-hydroxylase, beta polypeptide |
| NCBI Official Symbol: | P4HB�� |
| NCBI Official Synonym Symbols: | DSI; GIT; PDI; PHDB; PDIA1; PO4DB; PO4HB; PROHB; ERBA2L; P4Hbeta�� |
| NCBI Protein Information: | protein disulfide-isomerase; p55; protocollagen hydroxylase; prolyl 4-hydroxylase subunit beta; collagen prolyl 4-hydroxylase beta; thyroid hormone-binding protein p55; glutathione-insulin transhydrogenase; cellular thyroid hormone-binding protein; protein disulfide isomerase-associated 1; protein disulfide isomerase/oxidoreductase; protein disulfide isomerase family A, member 1; procollagen-proline, 2-oxoglutarate 4-dioxygenase (proline 4-hydroxylase), beta polypeptide |
| UniProt Protein Name: | Protein disulfide-isomerase |
| UniProt Synonym Protein Names: | Cellular thyroid hormone-binding protein; Prolyl 4-hydroxylase subunit beta; p55 |
| UniProt Gene Name: | P4HB�� |
| UniProt Entry Name: | PDIA1_HUMAN |
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