The Human Phosphodiesterase 10A (PDE10A) ELISA Kit is a highly sensitive and specific assay that is designed for the accurate detection of PDE10A levels in human samples such as serum, plasma, and cell culture supernatants. This kit provides reliable and reproducible results, making it ideal for various research applications.PDE10A is an enzyme that plays a crucial role in regulating the levels of cyclic AMP and cyclic GMP, which are important signaling molecules in various cellular processes. Dysregulation of PDE10A has been implicated in a range of diseases, including neurological disorders such as Huntington's disease and schizophrenia, making it a valuable biomarker for studying these conditions and developing potential therapies.
With its high sensitivity and specificity, the Human Phosphodiesterase 10A (PDE10A) ELISA Kit is a powerful tool for researchers looking to investigate the role of PDE10A in health and disease. Order yours today to advance your research efforts in this important field.
Product Name:
Human Phosphodiesterase 10A (PDE10A) ELISA Kit
Product Code:
HUDL02226
Size:
96 Assays
Target:
PDE10A
Synonyms:
cAMP and cAMP-inhibited cGMP 3',5'-cyclic phosphodiesterase 10A
Detection Method:
Sandwich
Range:
0.312-20ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
83-100
91
EDTA plasma (n=5)
91-102
96
Heparin plasma (n=5)
89-102
95
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
