Human PIN4 Recombinant Protein (RPPB2115)
- SKU:
- RPPB2115
- Product Type:
- Recombinant Protein
- Species:
- Human
- Uniprot:
- Q9Y237
- Research Area:
- Enzymes
Frequently bought together:
Description
| Product Name: | Human PIN4 Recombinant Protein |
| Product Code: | RPPB2115 |
| Size: | 20µg |
| Species: | Human |
| Target: | PIN4 |
| Synonyms: | Peptidyl-prolyl cis-trans isomerase NIMA-interacting 4, Parvulin-14, Par14, hPar14, Parvulin-17, Par17, hPar17, Peptidyl-prolyl cis-trans isomerase Pin4, PPIase Pin4, Peptidyl-prolyl cis/trans isomerase EPVH, hEPVH, Rotamase Pin4, PIN4, EPVH, MGC138486. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile filtered colorless solution. |
| Formulation: | PIN4 solution (1mg/ml) containing 20mM Tris-HCl buffer (pH8.0), 10% glycerol, 1mM DTT and 0.1mM PMSF. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Purity: | Greater than 85.0% as determined by SDS-PAGE. |
| Amino Acid Sequence: | MGSSHHHHHH SSGLVPRGSH MPMAGLLKGL VRQLERFSVQ QQASKMPPKG KSGSGKAGKG GAASGSDSAD KKAQGPKGGG NAVKVRHILC EKHGKIMEAM EKLKSGMRFN EVAAQYSEDK ARQGGDLGWM TRGSMVGPFQ EAAFALPVSG MDKPVFTDPP VKTKFGYHII MVEGRK |
| Biological Activity: | Specific activity is > 300 nmoles/min/mg, and is defined as the amount of enzyme that cleaves 1umole of suc-AAFP-pNA per minute at 25C in Tris-HCl pH8.0 using chymotrypsin. |
Peptidyl-prolyl cis-trans isomerase NIMA-interacting 4 (PIN4) is a peptidyl-prolyl cis/trans isomerase (PPIase) which interacts with NIMA and is vital for cell cycle regulation. PIN4 has 2 different isoforms: PAR14 and PAR17. Furthermore, Pin4 protein binds to double-stranded DNA under physiological salt conditions.
PIN4 produced in E.Coli is a single, non-glycosylated polypeptide chain containing 176 amino acids (1-156 a.a.) and having a molecular mass of 18.8kDa.PIN4 is fused to a 20 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
| UniProt Protein Function: | PIN4: Isoform 1 is involved as a ribosomal RNA processing factor in ribosome biogenesis. Binds to tightly bent AT-rich stretches of double-stranded DNA. Belongs to the PpiC/parvulin rotamase family. PIN4 subfamily. 2 isoforms of the human protein are produced by alternative promoter. |
| UniProt Protein Details: | Protein type:Nucleolus; Isomerase; Mitochondrial; EC 5.2.1.8 Chromosomal Location of Human Ortholog: Xq13 Molecular Function:protein binding |
| NCBI Summary: | This gene encodes a member of the parvulin subfamily of the peptidyl-prolyl cis/trans isomerase protein family. The encoded protein catalyzes the isomerization of peptidylprolyl bonds, and may play a role in the cell cycle, chromatin remodeling, and/or ribosome biogenesis. The encoded protein may play an additional role in the mitochondria. [provided by RefSeq, Dec 2009] |
| UniProt Code: | Q9Y237 |
| NCBI GenInfo Identifier: | 20139299 |
| NCBI Gene ID: | 5303 |
| NCBI Accession: | Q9Y237.1 |
| UniProt Secondary Accession: | Q9Y237,Q0D2H3, Q3MHV0, Q52M21, Q5HYW6, Q6IRW4, A8E0G6 B3KXM0, F5H1P5, |
| UniProt Related Accession: | Q9Y237 |
| Molecular Weight: | 14,179 Da |
| NCBI Full Name: | Peptidyl-prolyl cis-trans isomerase NIMA-interacting 4 |
| NCBI Synonym Full Names: | peptidylprolyl cis/trans isomerase, NIMA-interacting 4 |
| NCBI Official Symbol: | PIN4�� |
| NCBI Official Synonym Symbols: | EPVH; PAR14; PAR17�� |
| NCBI Protein Information: | peptidyl-prolyl cis-trans isomerase NIMA-interacting 4 |
| UniProt Protein Name: | Peptidyl-prolyl cis-trans isomerase NIMA-interacting 4 |
| UniProt Synonym Protein Names: | Parvulin-14; Par14; hPar14; Parvulin-17; Par17; hPar17; Peptidyl-prolyl cis-trans isomerase Pin4; PPIase Pin4; Peptidyl-prolyl cis/trans isomerase EPVH; hEPVH; Rotamase Pin4 |
| Protein Family: | Peptidyl-prolyl cis-trans isomerase |
| UniProt Gene Name: | PIN4�� |
| UniProt Entry Name: | PIN4_HUMAN |
