Human PKLR Recombinant Protein (RPPB2620)
- SKU:
- RPPB2620
- Product Type:
- Recombinant Protein
- Species:
- Human
- Uniprot:
- P30613
- Research Area:
- Enzymes
Description
| Product Name: | Human PKLR Recombinant Protein |
| Product Code: | RPPB2620 |
| Size: | 10µg |
| Species: | Human |
| Target: | PKLR |
| Synonyms: | PK1, PKL, RPK, pyruvate kinase isozyme R/L, Red cell/liver pyruvate kinase, PKRL |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile filtered colorless solution. |
| Formulation: | The PKLR protein solution (1mg/1ml) contains 20mM Tris-HCl buffer (pH8.0), 1mM DTT 0.2M NaCl and 10% glycerol. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid multiple freeze-thaw cycles. |
| Purity: | Greater than 90.0% as determined by SDS-PAGE. |
| Amino Acid Sequence: | MGSSHHHHHH SSGLVPRGSH MLTQELGTAF FQQQQLPAAM ADTFLEHLCL LDIDSEPVAA RSTSIIATIG PASRSVERLK EMIKAGMNIA RLNFSHGSHE YHAESIANVR EAVESFAGSP LSYRPVAIAL DTKGPEIRTG ILQGGPESEV ELVKGSQVLV TVDPAFRTRG NANTVWVDYP NIVRVVPVGG RIYIDDGLIS LVVQKIGPEG LVTQVENGGV LGSRKGVNLP GAQVDLPGLS EQDVRDLRFG VEHGVDIVFA SFVRKASDVA AVRAALGPEG HGIKIISKIE NHEGVKRFDE ILEVSDGIMV ARGDLGIEIP AEKVFLAQKM MIGRCNLAGK PVVCATQMLE SMITKPRPTR AETSDVANAV LDGADCIMLS GETAKGNFPV EAVKMQHAIA REAEAAVYHR QLFEELRRAA PLSRDPTEVT AIGAVEAAFK CCAAAIIVLT TTGRSAQLLS RYRPRAAVIA VTRSAQAARQ VHLCRGVFPL LYREPPEAIW ADDVDRRVQF GIESGKLRGF LRVGDLVIVV TGWRPGSGYT NIMRVLSIS |
| Biological Activity: | Specific activity: >0.1 unit/mg. One unit will form 1.0 umol of phospho(enol)pyruvate to pyruvate per minute at pH 7.5 at 37C. |
PKLR is a pyruvate kinase which catalyzes the transphosphorylation of phohsphoenolpyruvate into pyruvate and ATP. That is the rate-limiting step of glycolysis. PKLR gene encodes the L- and R-type isoenzymes through alternate splicing events controlled by different promoters. L-type isoform can also appear as a tetramer and is upregulated by glucose with implications in maturity-onset diabetes of the young.
PKLR Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 549 amino acids (47-574a.a.) and having a molecular wieght of 59.2kDa. The PKLR is fused to 21a.a. His-Tag at N-terminus and purified by proprietary chromatographic techniques.
| UniProt Protein Function: | PKLR: one of three rate-limiting enzymes in glycolysis. It catalyzes the transfer of a phosphate group from phosphoenolpyruvate (PEP) to ADP, yielding one molecule ATP and a pyruvate molecule, which is a central metabolic intermediate that can be used as a building block or oxidized further. There are several mammalian isozymes of pyruvate kinase encoded by different genes. The L type predominates in liver, and the M type in muscle and brain. Two alternatively spliced human isoforms have been described: the R- and L-types. |
| UniProt Protein Details: | Protein type:Nucleotide Metabolism - purine; Carbohydrate Metabolism - pyruvate; EC 2.7.1.40; Carbohydrate Metabolism - glycolysis and gluconeogenesis; Kinase, other Chromosomal Location of Human Ortholog: 1q21 Cellular Component: cytosol Molecular Function:pyruvate kinase activity Disease: Adenosine Triphosphate, Elevated, Of Erythrocytes; Pyruvate Kinase Deficiency Of Red Cells |
| NCBI Summary: | The protein encoded by this gene is a pyruvate kinase that catalyzes the transphosphorylation of phohsphoenolpyruvate into pyruvate and ATP, which is the rate-limiting step of glycolysis. Defects in this enzyme, due to gene mutations or genetic variations, are the common cause of chronic hereditary nonspherocytic hemolytic anemia (CNSHA or HNSHA). Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008] |
| UniProt Code: | P30613 |
| NCBI GenInfo Identifier: | 8247933 |
| NCBI Gene ID: | 5313 |
| NCBI Accession: | P30613.2 |
| UniProt Secondary Accession: | P30613,O75758, P11973, |
| UniProt Related Accession: | P30613 |
| Molecular Weight: | 58,494 Da |
| NCBI Full Name: | Pyruvate kinase PKLR |
| NCBI Synonym Full Names: | pyruvate kinase, liver and RBC |
| NCBI Official Symbol: | PKLR�� |
| NCBI Official Synonym Symbols: | PK1; PKL; PKR; RPK; PKRL�� |
| NCBI Protein Information: | pyruvate kinase PKLR |
| UniProt Protein Name: | Pyruvate kinase PKLR |
| UniProt Synonym Protein Names: | Pyruvate kinase 1; Pyruvate kinase isozymes L/R; R-type/L-type pyruvate kinase; Red cell/liver pyruvate kinase |
| Protein Family: | Pyruvate kinase |
| UniProt Gene Name: | PKLR�� |
| UniProt Entry Name: | KPYR_HUMAN |
