Human Platelet Activating Factor Acetylhydrolase 2 (PAFAH2) ELISA Kit
The Human Platelet Activating Factor Acetylhydrolase 2 (PAFAH2) ELISA Kit is a valuable tool for researchers studying the roles of PAFAH2 in various biological processes. This kit is specifically designed for the precise measurement of PAFAH2 levels in human serum, plasma, and cell culture supernatants.PAFAH2 is an important enzyme involved in the metabolism of platelet-activating factor (PAF), a lipid mediator with diverse biological functions. Dysregulation of PAFAH2 has been implicated in inflammatory diseases, neurological disorders, and cancer progression, highlighting its significance as a potential therapeutic target.
With its high sensitivity and specificity, the PAFAH2 ELISA Kit delivers reliable and reproducible results, making it an excellent choice for studies exploring the molecular mechanisms of PAFAH2 and its implications in human health and disease. Order yours today and accelerate your research efforts in understanding the role of PAFAH2 in biological processes.
Product Name:
Human Platelet Activating Factor Acetylhydrolase 2 (PAFAH2) ELISA Kit
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
