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Human PPME1 Recombinant Protein (RPPB2161)

SKU:
RPPB2161
Product Type:
Recombinant Protein
Species:
Human
Uniprot:
Q9Y570
Research Area:
Enzymes
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Description

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Product Name:Human PPME1 Recombinant Protein
Product Code:RPPB2161
Size:20µg
Species:Human
Target:PPME1
Synonyms:Protein phosphatase methylesterase 1, PME-1, FLJ22226, EC 3.1.1.
Source:Escherichia Coli
Physical Appearance:Sterile Filtered colorless solution.
Formulation:The PPME1 solution (0.5mg/ml) contains 20mM Tris-HCl buffer (pH 8.0), 1mM DTT, 0.1M NaCl and 20% glycerol.
Stability:Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.
Purity:Greater than 95% as determined by SDS-PAGE.
Amino Acid Sequence:MGSSHHHHHH SSGLVPRGSH MSALEKSMHL GRLPSRPPLP GSGGSQSGAK MRMGPGRKRD FSPVPWSQYF ESMEDVEVEN ETGKDTFRVY KSGSEGPVLL LLHGGGHSAL SWAVFTAAII SRVQCRIVAL DLRSHGETKV KNPEDLSAET MAKDVGNVVE AMYGDLPPPI MLIGHSMGGA IAVHTASSNL VPSLLGLCMI DVVEGTAMDA LNSMQNFLRG RPKTFKSLEN AIEWSVKSGQ IRNLESARVS MVGQVKQCEG ITSPEGSKSI VEGIIEEEEE DEEGSESISK RKKEDDMETK KDHPYTWRIE LAKTEKYWDG WFRGLSNLFL SCPIPKLLLL AGVDRLDKDL TIGQMQGKFQ MQVLPQCGHA VHEDAPDKVA EAVATFLIRH RFAEPIGGFQ CVFPGC

PPME1 catalyzes the demethylation and inactivation of protein phosphatase (PP2A), a multimeric phosphoserine/ threonine protein phosphatase related to growth inhibition and cell cycle arrest. PPME1 can demethylate PP2A catalytic subunit in vitro and okadaic acid treatment can inhibit this reaction. It is conserved from yeast to human and holds a motif found in lipases having a catalytic triad activated serine as their active site nucleophile.

PPME1 Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 406 amino acids (1-386) and having a molecular mass of 44.4 kDa.The PPME1 is fused to a 20 amino acid His-Tag at N-terminus and purified by proprietary chromatographic techniques.

UniProt Protein Function:PPME1: Demethylates proteins that have been reversibly carboxymethylated. Demethylates PPP2CB (in vitro) and PPP2CA. Binding to PPP2CA displaces the manganese ion and inactivates the enzyme. Belongs to the AB hydrolase superfamily. 3 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:Hydrolase; Motility/polarity/chemotaxis; EC 3.1.1.89; Protein phosphatase, regulatory subunit

Chromosomal Location of Human Ortholog: 11q13.4

Molecular Function:protein C-terminal methylesterase activity; protein phosphatase 2A binding; protein phosphatase binding; protein phosphatase inhibitor activity; protein phosphatase type 2A regulator activity

Biological Process: protein amino acid demethylation

NCBI Summary:This gene encodes a protein phosphatase methylesterase localized to the nucleus. The encoded protein acts on the protein phosphatase-2A catalytic subunit and supports the ERK pathway through dephosphorylation of regulatory proteins. It plays a role in malignant glioma progression. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2012]
UniProt Code:Q9Y570
NCBI GenInfo Identifier:47606055
NCBI Gene ID:51400
NCBI Accession:Q9Y570.3
UniProt Secondary Accession:Q9Y570,Q8WYG8, Q9NVT5, Q9UI18, B3KMU6, B5MEE7, J3QT22
UniProt Related Accession:Q9Y570
Molecular Weight:43,870 Da
NCBI Full Name:Protein phosphatase methylesterase 1
NCBI Synonym Full Names:protein phosphatase methylesterase 1
NCBI Official Symbol:PPME1��
NCBI Official Synonym Symbols:PME-1��
NCBI Protein Information:protein phosphatase methylesterase 1
UniProt Protein Name:Protein phosphatase methylesterase 1
Protein Family:Pectinesterase
UniProt Gene Name:PPME1��
UniProt Entry Name:PPME1_HUMAN

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