The Human ProADM (Proadrenomedullin) ELISA Kit is specifically designed for the precise measurement of Proadrenomedullin levels in human biological samples such as serum, plasma, and cell culture supernatants. This kit is characterized by its exceptional sensitivity and specificity, ensuring accurate and consistent results for a variety of research purposes.Proadrenomedullin is a critical peptide involved in various physiological processes, including vasodilation, immune modulation, and cell survival.
It is a valuable biomarker for studying conditions such as sepsis, cardiovascular diseases, and pulmonary disorders, making it a valuable tool for researchers and clinicians alike in understanding disease mechanisms and potential treatment strategies.Order the Human ProADM ELISA Kit from AssayGenie today and unlock insights into the role of Proadrenomedullin in human health and disease.
Product Name:
Human Pro-ADM (Proadrenomedullin) ELISA Kit
SKU:
HUES03474
Target:
Human Pro-ADM (Proadrenomedullin)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.47 pmol/mL
Detection range:
0.78-50 pmol/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human Pro-ADM. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human Pro-ADM and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human Pro-ADM, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human Pro-ADM. You can calculate the concentration of Human Pro-ADM in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
86-100
88-102
89-101
Average (%)
91
94
96
1:4
Range (%)
88-99
82-95
88-101
Average (%)
93
87
95
1:8
Range (%)
88-99
81-94
84-95
Average (%)
93
88
90
1:16
Range (%)
90-105
85-96
86-98
Average (%)
97
90
91
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
94-105
99
EDTA plasma (n=5)
95-107
101
Cell culture media (n=5)
94-109
100
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
2.43
7.94
19.47
2.37
7.2
18.19
Standard deviation
0.14
0.39
0.81
0.14
0.39
0.65
C V (%)
5.76
4.91
4.16
5.91
5.42
3.57
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human Pro-ADM concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human Pro-ADM in samples. No significant cross-reactivity or interference between Human Pro-ADM and analogues was observed.
