Human Procollagen III C-Terminal Propeptide (PIIICP) ELISA Kit
The Human Procollagen III C-Terminal Propeptide (PIIICP) ELISA Kit is a powerful tool for researchers looking to accurately measure levels of PIIICP in human samples such as serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit delivers reliable and reproducible results, essential for a wide range of research applications.PIIICP is a key biomarker for collagen synthesis and turnover, playing a crucial role in the formation and maintenance of connective tissues such as skin, bone, and cartilage.
Changes in PIIICP levels have been linked to various conditions including fibrosis, arthritis, and liver disease, making this ELISA kit an invaluable resource for studying these diseases and development of potential therapies.Don't miss out on the opportunity to enhance your research with the Human Procollagen III C-Terminal Propeptide (PIIICP) ELISA Kit from AssayGenie. Get your hands on this innovative tool and uncover new insights into collagen metabolism and related diseases.
Product Name:
Human Procollagen III C-Terminal Propeptide (PIIICP) ELISA Kit
Product Code:
HUDL02288
Size:
96 Assays
Target:
PIIICP
Synonyms:
P3CP, C-Propeptide Of Type III Procollagen, Procollagen III Carboxy Terminal Propeptide
Detection Method:
Sandwich
Range:
12.5-800pg/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
