Human PSMA5 Recombinant Protein (RPPB2200)
- SKU:
- RPPB2200
- Product Type:
- Recombinant Protein
- Species:
- Human
- Uniprot:
- P28066
- Research Area:
- Enzymes
Description
| Product Name: | Human PSMA5 Recombinant Protein |
| Product Code: | RPPB2200 |
| Size: | 5µg |
| Species: | Human |
| Target: | PSMA5 |
| Synonyms: | Proteasome subunit alpha type-5, Macropain zeta chain, Multicatalytic endopeptidase complex zeta chain, Proteasome zeta chain, PSMA5, PSC5, ZETA. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile Filtered colorless solution. |
| Formulation: | The PSMA5 solution (0.5mg/ml) contains 20mM Tris-HCl buffer (pH 8.0), 2mM DTT, 10% glycerol and 100mM NaCl. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Purity: | Greater than 90% as determined by SDS-PAGE. |
| Amino Acid Sequence: | MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSMFLT RSEYDRGVNT FSPEGRLFQV EYAIEAIKLG STAIGIQTSE GVCLAVEKRI TSPLMEPSSI EKIVEIDAHI GCAMSGLIAD AKTLIDKARV ETQNHWFTYN ETMTVESVTQ AVSNLALQFG EEDADPGAMS RPFGVALLFG GVDEKGPQLF HMDPSGTFVQ CDARAIGSAS EGAQSSLQEV YHKSMTLKEA IKSSLIILKQ VMEEKLNATN IELATVQPGQ NFHMFTKEEL EEVIKDI |
Proteasome subunit alpha type-5 (PSMA5) is a member of the peptidase T1A family. The proteasome is a multicatalytic proteinase complex which is distinguished by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. Proteasomes are spread throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. PSMA5 interacts with PLK1. The PSMA5 peptidase has a very wide-ranging specificity for cleavage of peptide bonds.
PSMA5 Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 277 amino acids (1-241) and having a molecular mass of 30.5kDa.PSMA5 is fused to a 36 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
| UniProt Protein Function: | PSMA5: The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity. The 26S proteasome consists of a 20S proteasome core and two 19S regulatory subunits. The 20S proteasome core is composed of 28 subunits that are arranged in four stacked rings, resulting in a barrel-shaped structure. The two end rings are each formed by seven alpha subunits, and the two central rings are each formed by seven beta subunits. The catalytic chamber with the active sites is on the inside of the barrel. PSMA5 interacts directly with the PSMG1-PSMG2 heterodimer which promotes 20S proteasome assembly. Up-regulated in colon cancer cell lines. Up-regulated in fetal Down syndrome (DS) brain. May be the target of the transcriptional activator NFE2L2. Expressed in fetal brain. Belongs to the peptidase T1A family. |
| UniProt Protein Details: | Protein type:EC 3.4.25.1; Proteasome complex; Protease Chromosomal Location of Human Ortholog: 1p13 Cellular Component: cytoplasm; cytosol; nucleoplasm; nucleus; proteasome complex; proteasome core complex Molecular Function:protein binding Biological Process: anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process; antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent; MAPKKK cascade; negative regulation of ubiquitin-protein ligase activity during mitotic cell cycle; positive regulation of ubiquitin-protein ligase activity during mitotic cell cycle; proteasomal ubiquitin-dependent protein catabolic process; protein polyubiquitination; regulation of amino acid metabolic process; regulation of mRNA stability; stimulatory C-type lectin receptor signaling pathway; T cell receptor signaling pathway; tumor necrosis factor-mediated signaling pathway; Wnt receptor signaling pathway, planar cell polarity pathway |
| NCBI Summary: | The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a member of the peptidase T1A family, that is a 20S core alpha subunit. Multiple alternatively spliced transcript variants encoding two distinct isoforms have been found for this gene. [provided by RefSeq, Dec 2010] |
| UniProt Code: | P28066 |
| NCBI GenInfo Identifier: | 38258905 |
| NCBI Gene ID: | 5686 |
| NCBI Accession: | P28066.3 |
| UniProt Secondary Accession: | P28066,Q3T1C1, Q6IBF7, B2R8F6, B4E2V4, |
| UniProt Related Accession: | P28066 |
| Molecular Weight: | 20,035 Da |
| NCBI Full Name: | Proteasome subunit alpha type-5 |
| NCBI Synonym Full Names: | proteasome subunit alpha 5 |
| NCBI Official Symbol: | PSMA5�� |
| NCBI Official Synonym Symbols: | PSC5; ZETA�� |
| NCBI Protein Information: | proteasome subunit alpha type-5 |
| UniProt Protein Name: | Proteasome subunit alpha type-5 |
| UniProt Synonym Protein Names: | Macropain zeta chain; Multicatalytic endopeptidase complex zeta chain; Proteasome zeta chain |
| UniProt Gene Name: | PSMA5�� |
| UniProt Entry Name: | PSA5_HUMAN |
