Human PTGR1 Recombinant Protein (RPPB2222)
- SKU:
- RPPB2222
- Product Type:
- Recombinant Protein
- Species:
- Human
- Uniprot:
- Q14914
- Research Area:
- Enzymes
Description
| Product Name: | Human PTGR1 Recombinant Protein |
| Product Code: | RPPB2222 |
| Size: | 20µg |
| Species: | Human |
| Target: | PTGR1 |
| Synonyms: | Prostaglandin reductase 1, PRG-1, 15-oxoprostaglandin 13-reductase, NADP-dependent leukotriene B4 12-hydroxydehydrogenase, PTGR1, LTB4DH, PGR1, ZADH3. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile filtered colorless solution. |
| Formulation: | The PTGR1 solution (0.5mg/ml) contains 20mM Tris-HCl buffer, pH7.5, 10% glycerol, 1mM DTT and 200mM NaCl. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Purity: | Greater than 90.0% as determined by SDS-PAGE. |
| Amino Acid Sequence: | MGSSHHHHHH SSGLVPRGSH MGSEFMVRTK TWTLKKHFVG YPTNSDFELK TAELPPLKNG EVLLEALFLT VDPYMRVAAK RLKEGDTMMG QQVAKVVESK NVALPKGTIV LASPGWTTHS ISDGKDLEKL LTEWPDTIPL SLALGTVGMP GLTAYFGLLE ICGVKGGETV MVNAAAGAVG SVVGQIAKLK GCKVVGAVGS DEKVAYLQKL GFDVVFNYKT VESLEETLKK ASPDGYDCYF DNVGGEFSNT VIGQMKKFGR IAICGAISTY NRTGPLPPGP PPEIVIYQEL RMEAFVVYRW QGDARQKALK DLLKWVLEGK IQYKEYIIEG FENMPAAFMG MLKGDNLGKT IVKA |
Prostaglandin Reductase 1 (PTGR1) is a member of the NADP-dependent oxidoreductase L4BD family. PTGR1 catalyzes the conversion of leukotriene B4 into its biologically less active metabolite, 12-oxo-leukotriene B4, thus being a primary step of metabolic inactivation of leukotriene B4. PTGR1 is highly expressed in the kidney, liver, and intestine but not in leukocytes.
PTGR1 Human Recombinant produced in E. coli is a single polypeptide chain containing 354 amino acids (1-329) and having a molecular mass of 38.6kDa.PTGR1 is fused to a 25 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
| UniProt Protein Function: | LTB4DH: Functions as 15-oxo-prostaglandin 13-reductase and acts on 15-oxo-PGE1, 15-oxo-PGE2 and 15-oxo-PGE2-alpha. Has no activity towards PGE1, PGE2 and PGE2-alpha. Catalyzes the conversion of leukotriene B4 into its biologically less active metabolite, 12-oxo-leukotriene B4. This is an initial and key step of metabolic inactivation of leukotriene B4. Belongs to the NADP-dependent oxidoreductase L4BD family. |
| UniProt Protein Details: | Protein type:EC 1.3.1.48; Oxidoreductase; EC 1.3.1.74 Chromosomal Location of Human Ortholog: 9q31.3 Cellular Component: cytoplasm Molecular Function:15-oxoprostaglandin 13-oxidase activity; zinc ion binding; 2-alkenal reductase activity Biological Process: response to toxin; cyclooxygenase pathway; arachidonic acid metabolic process; leukotriene metabolic process |
| NCBI Summary: | This gene encodes an enzyme that is involved in the inactivation of the chemotactic factor, leukotriene B4. The encoded protein specifically catalyzes the NADP+ dependent conversion of leukotriene B4 to 12-oxo-leukotriene B4. A pseudogene of this gene is found on chromosome 1. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2009] |
| UniProt Code: | Q14914 |
| NCBI GenInfo Identifier: | 23503081 |
| NCBI Gene ID: | 22949 |
| NCBI Accession: | Q14914.2 |
| UniProt Secondary Accession: | Q14914,Q8IYQ0, Q9H1X6, A8K0N2, B4DPK3, F5GY50, |
| UniProt Related Accession: | Q14914 |
| Molecular Weight: | 329 |
| NCBI Full Name: | Prostaglandin reductase 1 |
| NCBI Synonym Full Names: | prostaglandin reductase 1 |
| NCBI Official Symbol: | PTGR1�� |
| NCBI Official Synonym Symbols: | PGR1; ZADH3; LTB4DH�� |
| NCBI Protein Information: | prostaglandin reductase 1; PRG-1; 15-oxoprostaglandin 13-reductase; leukotriene B4 12-hydroxydehydrogenase; NADP-dependent leukotriene B4 12-hydroxydehydrogenase; zinc binding alcohol dehydrogenase domain containing 3 |
| UniProt Protein Name: | Prostaglandin reductase 1 |
| UniProt Synonym Protein Names: | 15-oxoprostaglandin 13-reductase (EC:1.3.1.48); NADP-dependent leukotriene B4 12-hydroxydehydrogenase (EC:1.3.1.74) |
| Protein Family: | Prostaglandin reductase |
| UniProt Gene Name: | PTGR1�� |
| UniProt Entry Name: | PTGR1_HUMAN |
