The Human PXR (Pregnane X Receptor) CLIA Kit is specifically designed for the accurate detection of PXR levels in human samples such as serum, plasma, and cell culture supernatants. This kit offers excellent sensitivity and specificity, ensuring consistent and reliable results for various research applications.PXR is a vital protein that plays a crucial role in drug metabolism and detoxification processes in the body. It is also involved in regulating the expression of various genes responsible for drug transport and metabolism, making it a key player in drug response and toxicity.
Studying PXR levels can provide valuable insights into drug interactions, drug metabolism, and potential toxicities, making this kit an essential tool for researchers in pharmacology, toxicology, and drug development. With its high-performance capabilities, the Human PXR CLIA Kit is a valuable asset for advancing research in the field of drug metabolism and pharmacokinetics.
Product Name:
Human PXR (Pregnane X Receptor) CLIA Kit
SKU:
HUES01264
Target:
Human PXR (Pregnane X Receptor)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human PXR. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human PXR and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human PXR, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human PXR. You can calculate the concentration of Human PXR in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
93-109
98-115
102-120
Average (%)
101
105
109
1:4
Range (%)
99-115
89-103
85-97
Average (%)
105
95
91
1:8
Range (%)
86-102
90-106
94-109
Average (%)
93
97
102
1:16
Range (%)
93-107
86-100
85-98
Average (%)
98
93
92
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
90-106
98
EDTA plasma (n=5)
95-111
101
Cell culture media (n=5)
92-104
98
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
104.2
206.55
908.49
111.55
203.46
981.23
Standard deviation
9.47
22.22
63.59
11.67
14.38
110.29
C V (%)
9.09
10.76
7.0
10.46
7.07
11.24
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human PXR concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human PXR in samples. No significant cross-reactivity or interference between Human PXR and analogues was observed.
